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- Methods Expression vector pCTLA 4/Ig was transfected into COS 7 with lipid reagent. The expression of fusion protein in the supernatant of the cell culture media was detected with double antibody sandwich ELISA. 方法 将pCTLA 4/Ig表达质粒转染COS 7细胞 ,双抗体夹心ELISA检测细胞培养上清中融合蛋白表达 ;
- Cloning of human pancreatic kallikrein gene and expression of fusion protein 人胰激肽释放酶基因的克隆及融合蛋白的表达
- Expression of fusion gene in transfected COS 7 cells was justified. 重组质粒转染COS 7细胞后经检测证实 ;该融合基因能在真核细胞中表达 .
- Keywords recombinant immunotoxins;liver cancer adhension peptides;pseudomonas exotoxin A;expression of fusion protein;functional identification; 免疫毒素;肝癌特异性结合肽;绿脓杆菌外毒素;融合表达;功能鉴定;
- Expression of fusion protein 融合蛋白表达
- Prokaryotic expression of structural domains of the Newcastle disease virus fusion protein and analyses of their antigenic epitopes. 新城疫病毒F蛋白结构域基因原核表达与抗原表位分析
- BVP22 can mediate intercellular trafficking of fusion protein in vitro and in vivo. 在体内外BVP22均能够介导与其融合的蛋白在细胞间发生穿梭。
- After treatment of MNC of CML and K562 with imatinib in vitro, the expression of DNA-PKcs protein was enhanced while tyrosine phosphorylation of bcr-abl fusion protein decreased. 伊马替尼体外作用于CML患者MNC及K562细胞后,DNA-PKcs蛋白表达量随着bcr-abl融合蛋白酪氨酸磷酸化水平的降低而升高。
- The recombination plasmid was transiently transfected into COS7 cells and the expression of the fusion protein was identified by RT PCR, immunofluorescent assay and sandwich ELISA. 以其瞬时转染COS7细胞 ,应用RT PCR、细胞免疫荧光技术 ,及夹心ELISA检测融合蛋白的表达。
- The fragment containing ubi-53 gene was inserted into pET-28a expression vector, expression of the fusion protein was induced by IPTG in E. Coli BL21 (DE3) . The fusion protein was identified by Western blot with a mouse Ab against bovine Ubiquitin. 将甜菜夜蛾的ubi-53基因克隆到原核表达载体pET-28a上，转化至大肠杆菌BL21（DE3）中，用IPTG进行诱导表达，用异源泛素单克隆抗体进行Western blot检测，证明了原核表达蛋白是目的蛋白。
- After transfection of pPTA1/Ig into COS 7 cells by DEAE dextran, expression of a secreting fusion protein was identified by affinity chromatography and sandwich ELISA assay with anti PTA1 mAb and HRP anti hIgFc mAb. 重组载体通过DEAE?dextran法转染COS?7细胞，经夹心ELISA及亲和层析、SDS?PAGE鉴定融合蛋白的表达及免疫学活性。
- After treatment with imatinib in mononuclear cell (MNC) of CML patients and K562 in vitro, expression of DNA-PKcs mRNA was detected by RT-PCR and DNA-PKcs protein level, tyrosine phosphorylation of bcr-abl fusion protein were detected by Western blot. 用伊马替尼体外作用CML患者的单个核细胞(MNC)及K562细胞后,用RT-PCR、Western blot方法分别检测DNA-PKcs mRNA和DNA-PKcs蛋白表达及bcr-abl融合蛋白的酪氨酸磷酸化水平。
- Then the plasmid was transfected into a mouse dendritic cell line, DC2.4, origining from C57BL/ 6 (B6) mouse, by LipofectAMINETM 2000. The expression of mBTIA mRNA and mBTLA-hIg fusion protein were determined by RT-PCR, ELBA and Western blot. 采用RT-PCR、ELISA和Western blot分别检测pmBTLA-hIg质粒转染DC中mBTLA基因mRNA的表达及细胞培养上清中mBTLA-hIg融合蛋白的表达;
- Objectives: To explore the functional property of fusion protein encoded by pGJA-P/VAX1. Methods: COS-7 cells were transfected with pGJA-P/VAX1 or pVAX1 using sofastTM transfect reagent. 目的:体外检测靶向融合防龋DNA疫苗pGJA-P/VAX1编码蛋白的生物学活性,研究蛋白是否具有靶向于抗原递呈细胞如树突状细胞的能力。 方法:pGJA-P/VAX1和pVAX1分别转染真核细胞COS-7,收集并浓缩细胞培养上清。
- Western Blotting of the two kind of fusion protein further displayed specific bands at the anticipated position, attesting that the fusion protein could react with antibody specifically. 用His-tag抗体进行Western-Blot检测，分别在预期的位置发现特异的条带,说明融合蛋白能与抗体进行特异性结合。
- An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
- I can think of no greater expression of defeatism. 这是我听到的最悲观的失败主义论调了。
- The strategies of fusion protein designing and its advantages and disadvantages in the bioprocessing of recombinant proteins, were introduced in this article. 介绍了构建融合蛋白的策略，优点及其存在的不足。
- There was an expression of discontent on her face. 她脸上有不满的表情。
- There is no way to harness the energy of fusion. 无法利用这种巨变能。
