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- Methods An open reading frame of human BSP was amplified by PCR method and was reconstructed into the eukaryotic expressive vector pIRES2-EGFP to construct the hBSP sense or antisense expressive vector . 方法以PCR的方法扩增人BSP的开放阅读框序列,与载体pIRES2-EGFP相连构成重组正反义表达质粒。
- Objective:To construct an eukaryotic expressing vector pIRES1neo/hFL and express hFL in COS 7 cell. 目的 :构建人FL真核表达载体 pIRES1neo/hFL ,观察其在COS 7细胞中的表达。
- Objective To construct endothelial cell-specific molecular-1(ESM-1) eukaryotic express vector,transfect it into human umbilical vein endothelial cells( HUVECs,ECV304) and express it in vitro . 目的 构建人内皮细胞特异性分子 1(ESM 1)真核表达载体 ,转染人脐静脉内皮细胞系ECV30 4 ,并在ECV30 4中获得表达。
- Objective:To construct and express a recombinant eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc fragment. 目的 :构建含人IL 2cDNA基因和免疫球蛋白Fc片段融合基因的真核表达载体pcDNA 3.;1IL 2 /Fc;并在真核细胞中表达;以期用于乙型肝炎病毒 (HBV)DNA疫苗的研究。
- AIM: To clone human IL-24 (hIL-24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein. 目的:克隆人IL-24基因并构建真核表达载体;转染Ca Ski细胞进行真核表达.
- Abstract: Objective To construct the eukaryotic expression vector which express the protein of VZV glycoprotein E in COS7 cells. [摘 要] 目的 构建水痘-带状疱疹病毒（VZV）糖蛋白E的真核表达载体并使其在COS7细胞中表达。
- Eukaryotic expression vector pCDNA3-AADC can be efficiently expressed in primary muscle cells. 含人类神经元AADC基因的真核表达重组体可在原代培养的骨骼肌细胞中有效的表达。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
- The constructed eukaryotic expression vector contained gB680 and pp65m fusion gene and expressed in COS-7 cells. 重组质粒含有gB680和pp65m融合基因,并能在COS-7细胞中表达。
- AIM: To clone human soluble TRAIL cDNA and construct its eukaryotic expression vector to prepare a condition for targeted gene therapy. 克隆人可溶性trail(strail)基因并构建肿瘤细胞特异性真核表达载体;为肿瘤基因治疗的靶向性奠定实验基础.
- Recombinant eukaryotic expression vector pCMV-Script/mito-MPG was constructed by molecule-cloning technique, and then transfected into A549/DDP cells. 构建pCMV-Script/mito-MPG重组真核表达载体; 脂质体将其转染至人非小细胞肺癌多药耐药细胞A549/DDP;
- Objective To construct a eukaryotic expression vector for expression of human neuropathy target esterase(NTE) esterase domain in mammalian cells. 目的构建人神经病变靶标酯酶(NTE)酯酶活力域(NESP)真核表达载体,并在细胞中表达。
- AIM: To clone rat CYP2J3 gene, construct Pcdna3.1(+)-CYP2J3 eukaryotic expression vector and detect its expression in endothelial cells of bovines. 目的:克隆大鼠CYP2J3基因并构建Pcdna3.;1(+)-CYP2J3真核表达载体;检测其在牛内皮细胞中的表达。
- ALR gene was also inserted to bicistronic plasmid p! RES2-EGFP to produce ALR, EGFP gene bicistronic eukaryotic expression vector (pIRES-EGFP/ALR). ALR和EGFP基因双顺反子表达载体，pIRES-EGFP/ALR。
- The eukaryotic expressing vector encoding human cbl is constructed and it expresses flag-cbl fused protein correctly in COS-7 cells. 成功构建了N 末端带flag标签的cbl真核表达载体 ;并使其在真核细胞COS 7中表达 .
- Methods: Eukaryotic expression vector plasmid containing AIRE cDNA was transfected into HeLa cells with FuGENE6 Transfection Reagent. 方法 :用 Fu GENE6转染试剂将含 AIRE c DNA的真核表达载体转染 He La细胞 ,经 G41 8筛选阳性克隆。
- So the HBx gene from genotype B hepatitis B virus , i.e., XB, was cloned into the eukaryotic expression vector pcDNA3.1/HisC in EcoR? and Xho? 为此将B基因型乙型肝炎病毒X基因克隆到pcDNA3.;1/HisC真核表达载体(重组载体为pcDNA3
- Objective To construct eukaryotic expression vector carrying human Apoptosis-inducing factor(AIF) gene fragment,pcDNA3.1(+)/AIF,and research the gene expression and function. 目的构建人凋亡诱导因子(AIF)基因功能片段的真核表达载体pcDNA3.;1(+)AIF;并初步研究AIF在细胞凋亡中的作用。
- Objective: To construct eukaryotic expression vector pSecTag2/HygroB-DAF of human decay accelerating factor (DAF) and transfect NIH/3T3 cells after encapsulated by chitosan. 目的:构建人补体衰变加速因子(DAF)真核表达载体pSecTag2/HygroBDAF,并利用壳聚糖组装成纳米微粒复合体,转染小鼠NIH/3T3成纤维细胞。
- AIM To construct an eukaryotic expression vector containing the coding region of human full length MUC1 gene and to detect its expression in COS 7 cells. 目的 构建含人 MUC- 1全长 c DNA序列的真核表达载体 ;并观察其在 COS- 7细胞中的表达 .
