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- Validation of a Rice Specific Gene, Sucrose Phosphate Synthase, Used as the Endogenous Reference Gene for Qualitative and Real-Time Quantitative PCR Detection of Transgenes. 一种水稻特异基因-蔗糖磷酸合成酶基因的确定,用作定性和实时定量PCR检测转基因的内标准基因。
- Endogenous reference gene 内源特异参照基因
- Results In PCR, the primer for internal reference gene, content of dNTP, and runing cycles affect the products much than the annealing temperature. 结果在pcr反应中,扩增内参照和目的基因的引物比例、底物浓度以及pcr反应的循环次数对产物的影响较大,而退火温度的影响较小。
- The results of this study will provide basis for GAPDH used as a reference gene to analyze the duck gene quantitatively. 研究结果为鸭GAPDH作为内参基因用于鸭相关基因定量表达分析奠定了基础.
- Don't refer to this matter again, please. 请不要再提这件事了。
- The grain-specific endogenuous reference genes w ere amplified successfully when using DNAs extracted with modified CTAB method. 0.5% RRS and 0.5% Bt? 176 Maximaizer could be identified by PCR detecting c amv 35? 改良CTAB法制备的DNA用作PCR模板均可扩增出内参照基因 ,PCR扩增camv35S启动子可检测出含量为 0 5%25的RRS和Bt176Maximaizer,而PCR扩增nos终止子可检测出含 1%25RRS的食品样品。
- This reference book is very useful to our study. 这本参考书对我们的研究很有用。
- Is this reference book of any use to me? 这本参考书对我有用处吗？
- I like to have my reference book within my reach. 我喜欢把参考书放在随手拿得到的地方。
- Tony used no reference books in class. 托尼上课时不使用参考书。
- Evidently shipowner to bear expense refer. 很明显船主应承担此费用。
- There is no further reference to him in her diary. 她的日记中没有进一步提到他。
- He spoke in reference to the cowboys. 他谈了有关牛仔的事。
- The book is used as a reference book. 这本书被用来做参考书。
- All such books were bought for reference. 所有这些书都是为参考而买的。
- No. we normally refer to metric ton. 不，我们通常指公吨。
- ARI can promote the production of endogenous NGF. ARI可促进 NGF的产生
- Botanists refer this flower to the rose family. 植物学家将这花归入蔷薇科。
- For further details, please refer to page 426. 详情请看四百二十六页。
- He keeps the original receipt for reference. 他保留正本(原始的)收据以便参考。
