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- The separation of primary mouse embryonic fibroblast (PMEF) and human embryonic fibroblast (HEF). 小鼠胚胎成纤维细胞(PMEF)和人胚胎成纤维细胞(HEF)的分离培养
- Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast(MEF) cells for isolation and culturing of embryonic stem(ES) cells. 目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
- Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization. 方法取大鼠胚胎分离成纤维细胞,分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
- The virus was continually inoculated 5 generations onto rabbit kidney cell and chicken embryonic fibroblast cell (CEF) and induced typical cytopathic effect (CPE). The typical CPE cloud be seen when culturing the virus on RK-13 cell . PK-15cell . 该分离毒株在兔肾原代细胞上(RK)上和鸡胚成纤维细胞(CEF)上连传5代均出现典型细胞病变，再接种RK-13细胞、BHK-21细胞、Vero细胞、PK-15细胞和143 TK~-细胞均出现典型细胞病变，在RK-13细胞上的感染滴度为10~(6.;52)TCID_(50)/0
- Embryonic fibroblast cells(EFCs) 胚胎成纤维细胞
- A characteristic cytopathic effect of CMV presented in a culture of human embryonic fibroblasts with conjunctival swab. 这些有病变反应之细胞经免疫萤光染色检定，对巨细胞病毒单株抗体染色呈阳性反应。
- Zhou JX, Liu D, Zheng KL, Deng CH, Wen RQ, Tang LX, Ma CJ.Culture conditions of mouse embryonic fibroblasts and preparation of feeder layer. 关键词：胚胎成纤维细胞；
- Feeder cells with murine embryonic fibroblasts(MEF) were better than that with human embryonic fibroblasts(hEF), or STO or bovine embryonic fibroblasts (bEF). MEF、STO、bEF和hEF可提高人原始生殖细胞(PGCs)的贴壁率,能促进PGCs源的人类胚胎干细胞(ES)的分离效率; 以MEF作为饲养层效果较好。
- Preliminary proteome analysis of mouse embryonic fibroblast conditioned medium 小鼠胚胎成纤维细胞条件培养液的蛋白质组学初步分析
- Keywords Embryonic fibroblast cells（EFCs）;Islet culture;Islet transplant;Rat; 关键词胚胎成纤维细胞;胰岛培养;胰岛移植;大鼠;
- Methods Neomycin gene was transfected into human embryonic fibroblasts (HEF) by liposome mediated method. Using this transfected HEF as feeder layer, ES cells were transfected with AANAT gene. 方法 将外源性neo基因转染人胚胎成纤维细胞 ,作为饲养层细胞用于ES细胞转基因后筛选及增殖过程的培养。
- RESULTS: Under the optical microscope, mouse embryonic fibroblasts were irregular, rapidly grew, passaged at 1:4.Following mitomycin C treatment, cells slowly adhered and could not proliferate. 结果：光镜下小鼠胚胎成纤维细胞呈不规则形，生长较快，传代比例为1:4，经丝裂霉素C处理后细胞贴壁较慢，失去分裂增殖能力。
- The scheme is still in its embryonic stage. 她脱颖而出成为著名艺术家。
- An embryonic nation, not yet self-governing. 一个早期的国家，还没有自治
- Following the embryonic stage of development. 胚胎后期的在胚胎发育期之后的
- One example of this kind of cell is the fibroblast. 例如成纤维细胞就属于这种细胞。
- mouse embryonic fibroblasts (MEFs) 小鼠胚胎成纤维细胞
- Effect of mouse embryonic fibroblast feeder layer and liver matrix of rabbit on the activities of LDH and ACP in cultured cells from schistosomula Schistosoma japonicum 鼠胚胎成纤维细胞饲养层及其与肝基质联合对日本血吸虫童虫培养细胞LDH、ACP影响的研究
- Human Fibroblast surface protein，FSP Elisa K... 人纤维母细胞表面抗原（FSP）试剂盒；
- Effects of mouse embryonic fibroblast feeder layers and combined of mouse embryonic fibroblast and collagen of rat tail on adhesion of cultured cells from Schistosoma japonicum 鼠胚胎成纤维细胞饲养层及与鼠尾胶联合促日本血吸虫培养细胞贴壁作用的研究
