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- The Pseudorabies virus (PRV) Fa strain gC gene was subcloned into Adenovirus shuttle vector pDC316 through double enzymatic digestion. 通过双酶切将伪狂犬病毒(Pseudorabies virus,PRV)Fa株gC囊膜糖蛋白基因片段亚克隆到5型腺病毒AdMax系统穿梭质粒pDC316中,得到pDC316-gC。
- Double enzyme digestion 双酶切
- The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes. 酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
- Epithelial depuration was carried out by integration of burning and enzyme digestion. 细胞形态观察和电镜检测鉴定细胞。
- Both enzyme digestion and alkali treatment are effective methods of cell rupture, but each has a major disadvantage. 酶消化法和碱处理法都是细胞破碎的有效方法，但是也都有各自的缺点。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- Protein hydrolysate preparation from Maixi carp was studied with double enzyme hydrolysis. 研究了双酶法制备麦溪鲤蛋白水解液。
- The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion. 将所获片段克隆入pGEN T-Easy载体，EcoR I酶切鉴定。
- Methods By tryosin and DNA enzyme digest methods, we observed the human beings chorial trophcytes with light microscope and electronic microscope. 方法应用胰蛋白酶／DNA酶消化法进行消化，经光镜和电镜观察。
- Extract hemoglobin from the fresh goose plasma, abstract and get out the hemoglobin peptide using the method of step-by-step hydrolysis of the double enzyme (dispase and caroid). 从新鲜的鹅血中提取血红蛋白,采用双酶水解法,分离提取血红蛋白肽。
- The recombination vector that was named as pCAM-P was obtained based on the tests of PCR and enzyme digestion of the vector. 2. PCR检测、质粒酶切分析结果表明获得了Pto与pCAMBIA1300的重组子，命名为pCAM-P。
- SCs were isolated by warm enzyme digestion, tissue culture, cold enzyme digestion of substep and cold enzyme digestion of combine. 雪旺细胞的分离采用了组织块法、热酶消化法、冷酶分步消化法和冷酶联合消化法。
- Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。
- BACKGROUND: At present, the preparation methods of acellular vascular matrix include enzyme digestion and decontaminant. 背景：目前脱细胞血管基质的制备方法有酶消化法和去污剂法。
- The cells were cultured by explant culture and enzymatic digestion. 组织化学染色和异种移植。
- Alkali treatment is the antithesis of enzyme digestion, for it is harsh rather than gentle, nonselective rather than specific, and cheap rather than expensive. 碱处理法和酶消化法相反，反应激烈，不具选择性，而且较便宜。
- Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 结果 1、对重组载体的酶切鉴定和测序结果证实，在pS2-DTA中EWS-FLI-1特异性结合序列(ACCGGAAGT)位于白喉毒素A链基因上游。
- Results 1.Both GAP-43 ORF gene fragment and the recombinant AAV-EGFP-GAP-43 plasmid were verified by enzyme digestion and DNA sequencing for correct reading frame and orientation. 结果:.;1
- Methods Exon 7 of SMN was detected in 15 cases with SMA and 33 cases of nomal control with polymerase chain reaction-restriction enzyme digestion analysis. 方法用聚合酶链反应-酶切技术对15例SMA病人及33例正常对照的外显子7进行检测，明确有无缺失。
- Then insert the fusion gene CTB-ureB into the vector pUC19. The recombinant plasmid was identified with PCR and enzyme digestion, and was confirmed by sequence analyze. CTB、ureB基因的融合及克隆：采用高保真PCR技术，分别从质粒pGEM-CTB和幽门螺杆菌基因组DNA中扩增出CTB和cagA基因，然后通过PCR法融合两个基因，最后将CTB-ureB融合基因插入载体pUC19，PCR筛选、酶切鉴定阳性克隆，进行DNA序列分析验证。
