Derivatization of FLX and NFLX with NBD-COC1 and DBD-COC1.As the fluorescent labeling reagents, NBD-COC1 and DBD-COC1, both of which has an acyl halide group, were selected to derivatize the amines.

• NBD-COC1的氟西汀和去甲氟西汀衍生物(NBD-FLX和NBD-NFLX)的最大荧光激发和发射波长分别为478±2nm和537±2nm，DBD-COC1的氟西汀和去甲氟西汀衍生物(DBD-FLX和DBD-NFLX)的最大荧光激发和发射波长分别为454±2nm和562±2nm。