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- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- DNA dot blot analysis showed that 73. 4% of resistant plants contained GNA and hpt genes. Southern blot analysis confirmed the integration of GNA gene in the genome of transgenic rice. DNA点杂交检测表明73.;4%25的抗性植株同时含有GNA基因和hpt基因;Southern杂交分析进一步证实了GNA基因在转基因水稻基因组中的整合
- Apply the PCRDIG probe to quickly detect the SRY gene by DNA dot blotting. PCR-DIG探针斑点杂交法快速检测SRY基因
- The results showed that, positive signals of DNA Dot blots for V-sis, c-erb B-2 , V-abl, H-ras, K-ras and c-myc are found. Among them the signals of c-sis and c-erb B-2 are stronger than the others. 结果发现,V-sis、c-erbB-2、V-abl、H-ras、K-ras和c-myc质粒DNA的斑点出现阳性杂交信号,其中V-sis、c-erbB-2的斑点信号最强。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis进行整合,经G418筛选得到25个高拷贝转化子,经DNA斑点试验和DNA测序证明外源基因E2稳定地整合到P.;Pastoris染色体中。
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- Regenerated plants with kanamynic resistantance were obtained. PCR , PCR-Southern blot analysis , southern dot blot analysis of lettuce DNA confirmed that adw gene had been integrated into the plant genome. 细胞核载体PB-adw、PBG-adw均采用农杆菌介导法将adw导入莴苣,细胞核转化获得了生长良好的抗性莴苣植株,经PCR、PCR-Southern、southern斑点杂交分析证实,adw基因已整合到莴苣基因组中。
- In Q group the gene expressions of DNA polymerase beta, EGFR and c-myc detected by RNA dot blot array were 6.5 folds, 7 folds and 2.2 folds down-regulated respectively, and the wtp53 was 2.5 folds up. Q组的DNAPolp,EGFR及e一mye的RNA斑点TLe扫描数值分别比对照组下调了6.;5倍,7倍,2
- The results of reverse spot hybridization for both duck hepatitis B virus DNA (DHBV DNA) and special DNA polymerase were compared with those by dot blot for DHBV DNA and electromicroscopy for DHBV particles. 本文将逆向斑点杂交同时检测鸭乙型肝炎病毒DNA(DHBV DNA)及其特异DNA多聚酶(DNAp)的结果,与斑点杂交检测DHBV DNA和电镜检测DHBV颗粒的结果作了比较。
- Dot blot hybridization with the two probes on Nitrocellulose Membranes showed the positive results for PPV DNA from different resources and PUP recombinant plasmid,but negative for the nucleic acid samples obtained from a series of control virus. 分别对不同来源的猪细小病毒DNA及PUP重组质粒于硝酸纤维素膜上打点杂交,免疫呈色后均为阳性反应,而对照的猪瘟病毒、乙型脑炎病毒、伪狂犬病毒、PK-15细胞的核酸均为阴性反应。
- Dot blot hybridization with the two probes showed the positive result for PPV DNA,but negative for the nucleic acid samples obtained from Hog cholera virus,Pseudorabies virus,Japanese B Encephalitis virus and PK 15 cells. 对猪细小病毒DNA进行斑点杂交,两种探针均为阳性,而对照的猪瘟病毒、猪伪狂犬病毒、乙型脑炎病毒及PK-15细胞的核酸均为阴性。
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶链反应(PCR)结合反向斑点杂交(RDB)技术。
- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot杂交技术定量研究3组胃粘膜ET?1、NOSmRNA表达。
- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 应用完整细胞原位斑点印迹杂交技术检测c-myc、野生型p53(wtp53)、p16和EGFR的基因表达;
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特异性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的:探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- After random labeling with DIG, RNA dot blot was proceed to test the expression of this gene under different growth condition. 用地高辛随机引物标记试剂盒对该片段进行标记后,对不同水分和硫营养条件下提取的小麦根系总RNA 进行斑点杂交。