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- DNA shuffling performed well in the evolution of DNA, protein and organism. 无论DNA、蛋白质还是生物体的进化,DNA改组都有十分突出的表现。
- DNA shuffling has been one of the most effective directed evolution methods since reported in 1994. 摘要自1994年首次提出至今,DNA改组已经成为定向进化最为有效的方法之一。
- Here we gave reviews in molecular evolution, especially the technology of DNA shuffling and its main applications and development. 本文就分子进化,尤其是DNA改组的技术、方法以及近年的主要研究进展进行综述。
- In the past few years, many new protocols for directed evolution were developed on the basis of oligonucleotide -directed random mutation, error-prone PCR and DNA shuffling. 最近几年,在过去常用的寡核苷酸介导的随机突变、易错PCR和DNA改组等方法的基础上又出现了一些新的定向进化方法。
- Over the twenty years, it has branched into three characteristic strategies: splicing by overlapping extension (SOE), jumping polymerase chain reaction (JPCR) and DNA shuffling. 经过二十多年的发展,该技术已形成三个各具特色的方法分支:重叠延伸拼接、跳跃PCR和DNA重排。
- Directed Evolution of Hirudin by DNA shuffling DNA改组技术在水蛭素实验进化中的应用
- DNA shuffling and its applications in vaccines DNA改组及其在疫苗研制中的应用
- The Application of Directed Enzymes Evolution in Vitro by DNA Shuffling 同源基因拼接技术在酶体外进化中的应用
- APPLICATIONS OF DNA SHUFFLING AND GENOME SHUFFLING IN METABOLIC ENGINEERING DNA及基因组改组在代谢工程中的应用
- DNA shuffling and Its Application on Genetically Engineering Vaccines 技术及其在基因工程疫苗中的应用
- Study on DNA Shuffling Method for Changing the Promoter Activity of aac C1 Gene 利用DNA改组技术改造aac C1基因启动子活性的研究
- Keywords Hepatitis C Virus;DNA Shuffling;Reassembly via PCR;Directed Evolution; 肝炎病毒;丙型;DNA改组;重聚PCR;定向进化;
- Keywords DNA shuffling;phage disp lay;interferon;expression;purifica tion; DNA改组;噬菌体表面呈现技术;干扰素;表达;纯化;
- Construction of phage-displayed library by DNA shuffling and the screening of potent hirudin variants 噬菌体改组库的构建及高活性水蛭素变异体的筛选
- One of the orderlies, Levy was shuffling the cards. 一个叫莱维的勤务兵正在洗牌。
- DNA fingerprinting can help the police to find the criminals. DNA指纹术有助於警察发现罪犯。
- DNA shuffling DNA改组
- Keywords anti-CD3 reshaped single chain fragment antibody(ScFv);affinity;megaprimer PCR;site-directed mutagenesis;DNA shuffling;random mutation;phage antibody library;molecular modeling; 抗CD3改型单链抗体;亲和力;巨型引物PCR;定点诱变;DNA改组;随机突变;噬菌体抗体库;分子模拟;
- Shuffling the lineup hasn't done the trick. 调动先发选手的打击顺序并没有产生好结果。
- Some one was shuffling by on the sidewalk. 外面的人行道上有人拖着脚走过。