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- DNA directed DNA polymerase(DNA dependent DNA poly-merese) DNA指导性DNA聚合酶
- DNA directed DNA polymerase DNA dependent DNA poly-merese
- RNA directed DNA polymerase 核糖核酸指导的脱氧核糖核酸聚合酶, RNA指导的DNA聚合酶
- FastStart Taq DNA Polymerase, December ed. 酸外切酶活性( Roche Package Insert.
- Methods Mutation analysis of TH gene was performed in 5 probands with AR-DRD and 2 sporadic patients with DRD by use of polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) combining DNA direct sequencing. 方法 应用聚合酶链反应 -单链构象多态性技术和 DNA序列分析方法对 5个 AR- DRD家系的先证者和两例散发DRD患者进行 TH基因突变分析。
- Using chromosome DNA of Lactobacillus casei 34103 as template, thy A (Thymidylate synthase) gene was amplified by PCR with pfu DNA polymerase. 以干酷乳杆菌L.;casei34103染色体DNA为模板,利用PCR技术扩增胸苷酸合成酶(Thymidy-1atesynthase,fhyA)基因,回收纯化。
- Telomerase is RNA dependent DNA polymerase, acts to synthesize DNA repeat fragment of telomeres , which is associated with immortalization of cells. 端粒酶是一种RNA依赖的DNA多聚酶,可以合成端粒DNA的重复片段与细胞永生化密切相关。
- First, this term is often used to describe both the viral subtype (A,B,C,D, etc) as well as drug resistance mutations in the HBV DNA polymerase gene. 首先,这个术语经常被用来描述病毒亚型(A,B,C,D等)以及HBV DNA多聚酶基因中出现的耐药性突变。
- Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification. 用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA,直接转化大肠杆菌。
- DNA directed RNA polymerase(DNA dependent RNA poly-merase) DNA指导性RNA聚合酶
- Detection of keratin 5 gene point mutation in a family with Weber Cockayne EBS by PCR and direct DNA sequencing. PCR-DNA直接测序检测1例单纯型大疱性表皮松解症Weber-Cockayne亚型(WC-EBS)患者角蛋白K5基因点突变
- In contrast, direct DNA sequencing requires only a single sample from the proband. 相比之下,直接测序只需要被检测者的血样 。
- Phylogeny within DNA polymerase X family requires a re examination as the expansion of family members,particularly considering the enter of two entomopoxvirus(EPV) members. 随着DNA聚合酶X家族成员数量的增加 ,特别是两个昆虫痘病毒 (entomopoxvirus ,EPV)成员的加入 ,家族内部的系统发育关系需要重新检查。
- Further studies showed that DNA replication fidelity is decreased and there is an alteration of DNA polymerase spectrum when mammalian cells are attacked by MNNG. 因此,我们推测,哺乳细胞中低保真度的跨损伤DNA聚合酶可能参与了MNNG诱导的哺乳细胞非定标突变。
- A system used for the determination of fidelity of DNA polymerase in PCR was developen in E.coli and was used to determine the fidelity of FD DNA polymerase in PCR amplication. 摘要 在大肠杆菌中建立了一套用于测定 DNA聚合酶在PCR过程中复制精确性的系统,并测定了耐热FD DNA聚合酶在PCR扩增过程中的复制精确性。
- In molecular biology, processivity is a measure of the average number of nucleotides added by a DNA polymerase enzyme per association/disassociation with the template. 应该说PCR其他的技术层次都已经很完善了,唯一的瓶颈是DNA聚合酶的持续合成能力(processivity)。
- The methods include using Agr obacterium-mediated transformation, electroporation, gene gun bombardme nt and polyethyleneglycol (PEG)-mediated direct DNA transfer. 果树基因转化方法主要包括农杆菌介导法、电激法、基因枪法、PEG转化法等。
- Lamivudine, and most nucleoside analogues, acts by suppression of viral replication through competitive inhibition of reverse transcriptase and DNA polymerase. 拉米还有大多数的核苷类似物,通过竞争性地阻断翻转录酶和DNA聚合酶来抑制病毒复制。
- Methods:ABO blood groups were identified by serological tests. B(A) alleles were determined by PCR-SSP and direct DNA sequencing at exons 6 and 7 of ABO gene. 方法:用血清学血型方法、PCR-SSP法和ABO基因第6及第7外显子直接测序的方法对B(A)血型和B(A)型等位基因进行检测。
- The mutations were detected by direct DNA sequencing. Results All samples examined carried mtDNA A1555G mutation in 12SrRNA gene and G7444A mutation in COI/tRNASer(UCN) gene. 结果测序结果表明,此家系线粒体DNA12SrRNA基因中存在着A1555G突变,COI/tRNASer(UCN)基因中存在着G7444A突变。
