Co-cultures of mouse oocytes with pBR322 -HBV DNA plasmids were performed and the entering of HBV DNA into the oocytes and its integrating in host chromosomes were demonstrated using PCR, dot and Southern hybridizations and FISH techniques.
英
美
- 将小鼠卵母细胞与pBR322-HBV DNA质粒共培养,用斑点杂交、PCR、Southern杂交与FISH技术证实HBV DNA进入卵母细胞并在卵母细胞染色体上整合。