Callus and adventitious bud induction :The leave were cultured under the sunlight by using the improved liquid medium , MS+BA1+2, 4-D0.5. After two weeks, the bud differentiation rate was more than 1300%.

• 诱导外植体的生长与分化：利用改进的液体培养基MS+BA1+2，4-D0.；5，在自然光下进行培养。