Biological effect of rIL-12 on PBMC from blood donors was estimated by the methods of cytokine follow cytometry (CFC), tetramer staining, NK activation, non-specific and specific lymphocyte proliferation.

• 重组IL-12作用于献血员外周血单个核细胞(PBMC),分别应用细胞因子流式细胞(CFC)计数、Tetramer染色、NK杀伤实验、非特异性及特异性淋巴细胞增生反应等方法体外分析重组IL-12的生物学活性。