An indirect ELISA using protein VP2 which was expressed by Picha Pastoris as coating antigen was established to monitor antibodies level of pigs against PPV and its operation methods were optimized.

 
  • 通过酵母分泌表达获得的猪细小病毒VP2蛋白经透析纯化后,作为包被抗原初步建立了检测猪细小病毒抗体水平的间接ELISA方法,并对该方法进行了优化,用此优化方法检测了200份来自发病猪场的待检猪细小病毒阳性血清样品,并将其与华中农业大学的乳胶凝集试剂盒相比较,符合率达92.;6%25。
今日热词
目录 附录 查词历史