After sterilization and addition of NAD+ in the used substrate solution, the immobilized cells could be recycled for 10 times to maintain the conversion rate of ATP over 90%.

• 在前一批底物溶液灭菌和添加烟酰胺腺嘌呤二核苷酸的条件下,固定化酵母细胞可反复使用10次,ATP转化率稳定维持在90%25以上。