After drug-given, the serum sex hormone (E3, E2) and osteocalcin (BGP) levels were detected by radioimmunoassay, and the broadband ultrasound attenuation (BUA) of femur was tested with quantitative ultrasound (QUS) , the femur weight was calculated.

• 灌胃给药后,用放射免疫法测定大鼠血清雌三醇(E3)、雌二醇(E2)及骨钙素(BGP)水平,用定量超声法测定股骨超声振幅衰减(BUA),并测定大鼠股骨重量以计算股骨干重指数及灰重/干重。