A sensitive technique for the isolation of leptospirae consists of the intraperitoneal inoculation of young hamsters or guinea pigs with fresh plasma or urine.

• 将含有钩端螺旋体的新鲜血浆或尿液接种于幼龄豚鼠或金地鼠腹腔是分离钩端螺旋体的敏感方法。