A reversed phase high performance liquid chromatography with precolumn derivatization was developed for the simultaneous determination of 5-aminolevulinic acid(ALA) and glycine(Gly) in E.

• 建立了同时检测发酵液中5-氨基乙酰丙酸和甘氨酸的柱前衍生反相高效液相色谱法。