A quantitative competitive PCR(qcPCR) assay was used to detect CD80-CD86 mRNA levels in porcine alveolar macrophages(PAMs) during PCV2 infection through construction of their respective competitive depletion clones.

• 通过构建猪共刺激分子CD80和CD86的缺失cDNA竞争分子,用竞争PCR技术定量检测了猪圆环病毒2型(PCV2)感染后猪肺泡巨噬细胞(PAM)中CD80和CD86的mRNA水平,分析了PCV2感染对猪共刺激分子CD80和CD86的mRNA表达的影响。