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- Methods:PCR,denatur ing gradient gel electrophoresis (DGGE) atd DNA sequencing were used. 方法：采用多聚酶链反应（PCR），变性梯度凝胶电泳（DGGE）和DNA测序等方法对上海地区血友病甲基因突变进行检测。
- Denaturing gradient gel electrophoresis(DGGE) is an advanced molecular fingerprinting technique. 变性梯度凝胶电泳(DGGE)是近些年微生物分子生态学研究中的热点技术之一。
- The results showed that soil samples collected from same area had the high identity in DGGE bands patterns. 结果表明,采自同一地区的土壤样品，其DGGE图谱的相似性很高；
- Through physiological and biochemistry experiments and 16SrDNA sequence analysis, the two marine bacteria strains CHS and SY, were further identified as Pseudoalteromonas. 从分离到的217株细菌中,获得23株碱性蛋白酶酶活高的菌株,对其中两株海洋细菌CHS和SY菌株进行生理生化和16SrDNA序列分析,初步鉴定为交替假单胞菌。
- The DGGE band number fromthe subsoil samples was obviously more thanthat fromthe soil samples around the pond. 底泥样品的DGGE条带数明显多于池塘周边土壤样品。
- Results shown that the PCR product of bacterial nucleic acid, which was purified with modified CTAB method, obtained stablethe most bands in DGGE. 结果表明,改良CTAB法抽提获得细菌核酸的PCR产物在DGGE凝胶上获得的条带最多,结果稳定。
- What could you conclude from PCR/DGGE analysis of a simple that yielded one band by PCR and one band by DGGE?One band by PCR and for band by DGGE? 前者说明该条带具有固定的序列及片段大小相同，后者说明片段大小虽然相同，但序列还是存在差异。
- Some DGGE strip lack or increase showed that fungus group had changed differently in rhizosphere and non-rhizosphere soil. 真菌数量有所增加，种群发生了明显改变，表现为条带的缺失和增加。
- In this paper, techniques, such as Biomarker, SCSU, DNA renaturation experiment, DGGE, TGGE, ARDRA, T-RFLP, SSCP, PAPD and ERIC-PCR, commonly used in this field were introduced. 本文介绍了生物标志物法、SCSU、DNA复性分析、DGGE、TGGE、ARDRA、T-RFLP、SSCP、PAPD和ERIC-PCR 图谱分析等方法在土壤微生物群落结构研究中的应用。
- Methods Denaturant gradient gel electrophoresis (DGGE) was used to scan all DNA fragments of 3 genes mentioned above in AD group (15 cases) and elderly control group (241 cases). 方法 应用DNA变性梯度凝胶电泳方法对15例AD和241例老年对照组的上述3个基因的DNA片段进行扫描分析。
- Effects of different cropping system on diversity of soil bacterial community were studied by using denatured gradient gel electrophoresis (DGGE) analysis. 摘要 采用变性梯度凝胶电泳(DGGE )技术，研究了不同种植模式对土壤细菌群落多样性的影响。
- Results showed that the numbers and strength of the bands from DGGE profiles varied with the different cropping system, especially the distinguished bands were different. 结果表明，不同种植模式下DGGE图谱条带的数量及亮度有较大区别，且有几条特征性条带发生了明显的变化。
- This article reviewed the principle, merits and limitations of DGGE technique and also reviewed the latest research in the oral cavity with this novel technique. 下面就DGGE的原理、在口腔微生物研究中的应用、优缺点和应用前景作一综述。
- A method based on PCR was developed to amplify short tandem repeat(STR) in combination with denaturing gradient gel electrophoresis (DGGE) and silver dyeing. 应用聚合酶链反应扩增STR,经变性凝胶电泳,再用银染显色方法,分析苯丙氨酸羟化酶(PAH)基因内含子3中TCTA重复序列的多态性。
- In the paper,the principle of DGGE and its application in food microbiology were reviewed.The application of PCR-DGGE in fermented food research was illustrated outstandingly. 介绍了DGGE的基本原理及其在食品微生物学研究中的应用,着重阐述了PCR-DGGE在发酵食品研究中的应用进展。
- After PCR anplification of total community DNA using a specific primer set,why is it usually necessary to eithercloneor run DGGE on the products before sequencing them? 相同的条带表示分子量相同但可能序列不同，需通过DGGE检测证明是一种DNA序列。
- The results of DGGE and analysis of nucleotide sequences of 28S rDNA showed identical patterns and nucleotide sequences in the serotype A and D of C. neoformans var. neoformans, which were distinct from the serotye B and C of C. neoformans var. gattii. 经过DGGE和DNA序列分析,所有新生隐球菌新生变种(A、D型)具有一致的基因带型和序列,格特变种(B、C型)具有不同于新生变种的独特一致的带型和序列;
- Because of its objective, rapid and accurate assessment character, DGGE has extensively been used on assessment of the diversity and changes of bacterial species in the microbial population. 由于DGGE技术具有可靠性强、重现性高和方便快捷等优点,被广泛地应用于微生物群落多样性和动态性分析。
- denaturing gradient gel electrophoresis (DGGE) 变性梯度凝胶电泳
- denatured gradient gel electrophoresis(DGGE) 变性梯度凝胶电泳（DGGE）
