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- Methods Plasmids containing HPV-16 E6 or E7 were used to generate absolute standard curves. 方法将制备的含HPV-16E6和E7基因的质粒作为标准品,建立实时荧光定量PCR和RT-PCR方法,分别对3种宫颈癌细胞株进行HPV-16E6和E7基因的DNA和RNA拷贝数的定量检测。
- HPV16 E7 was expressed efficiently in HEK-293 and HeLa cells. 荧光显微镜下,转染后HEK-293细胞和HeLa细胞表达绿色荧光蛋白。
- In Large White, the BB genotype at E5 was favorable marker genotype for TNB and NBA, and the BB genotype at E7 was favorable marker genotype for TNB. 对于大白猪，E5位点BB基因型可作为在TNB和NBA性状上的标记基因型，E7位点BB基因型可以作为TNB性状的标记基因型。
- Unfortunately he won't be here tomorrow evening. 可惜的是他明晚不在这儿。
- He has a yen to be alone in a boat. 他渴望独自待在一条船上。
- It is necessary that he be sent there at once. 有必要马上派他到那里去。
- I think most of these obstacles can be surmounted. 我认为这些障碍大多数都是可以克服的。
- I think the work can be completed ahead of time. 我认为这项工作能提前完成。
- He likes to be sure of his bodily comforts. 他注重身体上的舒适。
- At this rate we won't be able to afford a holiday. 照这样下去，我们不会有时间／钱去度假的。
- Roughness of the skin can be caused by bad diet. 饮食不好可能引起皮肤粗糙。
- In order not to be late, she cut across the fields. 为了不迟到，她抄近路穿过田野。
- Stress can be the undoing of so many fine players. 紧张可能是这许多优秀选手失败的原因。
- The method seems good but it needs to be tried out. 这个方法似乎不错，但需要试验一下。
- Let your yea be yea and your nay be nay. 如实说你的想法。
- When will be the'96 Paralympics hold? 大赛何时举行的?
- Don't be unduly worried about the exam. 不要过分担心考试的事。
- Our brigade will be committed at dawn. 我们旅黎明就要投入战斗。
- A butterfly must not be confused with a moth. 不得将蝴蝶和飞蛾混同起来。
- I admit I could not be so far ben as Ivan. 我承认我不能像伊文一样受到赏识。
