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- viral nucleic acid analysis 病毒核酸分析
- The viral nucleic acid was ssDNA after digested with DNase,RNase and Sl nuclease,Analysis of viral polypeptides by SDS-PAGE revealed that the virion had four structural polypep-tides with MW 86 kD,79kD,70 kD and 25.5 kD. 用脱氧核糖核酸酶(DNase),核糖核酸酶(RNase)及二苯胺染色法对病毒核酸进行处理,证明该病毒核酸为脱氧核糖核酸,用Sl核酸酶(Slnucleasc)对该核酸进一步消化处理,进一步证实该病毒含单链DNA。 SDS-PAGE结果显示,该病毒含4条结构多肽,其分子量分别为86kD,79kD,70kD及25.;5kD。
- After reproduction of the viral nucleic acid the host cell usually undergoes lysis (LYSOGENY). 病毒核酸复制后寄主细胞通常要经历裂解过程(溶源状态)。
- Infection of a cell with purified viral nucleic acid, resulting in subsequent replication of the virus in the cell. 传染细胞被纯病毒核酸感染,从而引起病毒在细胞中的繁殖
- Infection of a cell with purified viral nucleic acid,resulting in subsequent replication of the virus in the cell. 传染细胞被纯病毒核酸感染,从而引起病毒在细胞中的繁殖
- Uncoating with its release of viral nucleic acid into the cytoplasm can be inhibited if the virion is covered by antibody. 如果病毒体被抗体覆盖,脱衣壳释放核酸这一步就会被抑制。
- The inert extracellular form of the virus, termed a virion, penetrates the host membrane and liberates the viral nucleic acid into the cell. 多数情况下,病毒核酸被宿主细胞核糖体翻译,继而产生复制和包装产生子代病毒所需的酶类。
- If, however, only the viral nucleic acid were necessary for replication, then only the P32 would be found in the cytoplasm. 可是如果病毒复制只需要核酸,那就只有P32能在细胞质中被发现。
- The complete fully assembled virus is termed the virion. This structure, the virion, serves to transfer the viral nucleic acid from one cell to another. 具有在细胞间转移病毒核酸能力的完整的病毒称作为病毒体。
- Besides, some other commonly used methods include the traditional method, Biolog method and nucleic acid analysis. 本文还将常用的研究微生物多样性的几种方法进行了比较。
- Methods By RT PCR DDH,RT nested PCR,RNA DDH,we detect the nucleic acid of HCV and deduce the specificity,sensitivity and stability of RT PCR DDH in viral nucleic acid detection. 方法 通过RT PCR DDH ,RT nested PCR和HCVRNA DDH 3种方法检测HCV核酸 ,推断RT PCR DDH技术检测病毒核酸的特异性、敏感性和稳定性。
- The viral nucleic acid contains information necessary for programming the infected host cell to synthesize virus-specific macromolecules required for the production of viral progeny. 病毒的核酸含有使感染细胞合成子代病毒所需的病毒特异性大分子的所有必需的信息.
- Viral nucleic acids were detected in the tissues of heart, kidney, lung, small intestine and bursa of Fabricius at PI 12 h. 结 果表明:用5 个LD50(经点眼滴鼻方式测定)的AIV SZ 株经点眼滴鼻途 径接种实验鸡后,攻毒组PI 12 h 时,即可以在心、肾、肺、小肠和法氏囊 检测到病毒核酸;
- Plasmid of HBV DNA dimer was transfected into a differentiated human hepatoma cell line(HepC2), and a stable HBV-producing cell line(z7-4) was set up which had capable of secreting HBcAg/HBeAg,viral nucleic acids and of releasing Dane-like particles. 用HBV DNA二聚体转染高度分化的人肝癌细胞株(HepG2),筛选出具有分泌病毒抗原、核酸以及释放42nm Dane颗粒能力的z7-4细胞株。
- The Nucleic Acid Analysis by DNA Chip Technique Based on Nuclease S1 Protection 基于核酸保护原理的 DNA芯片检测技术
- infection of a cell with purified viral nucleic acid,resulting in subsequent replication of the virus in the cell 传染细胞被纯病毒核酸感染,从而引起病毒在细胞中的繁殖
- Property of Viral Nucleic Acid from the Virus Causing the Trembling disease in Eriocheir sinensis 引致河蟹颤抖病的病毒的核酸定性
- Amino acid analysis of ACE inhibitory peptides. 血管紧张素转换酶抑制肽的氨基酸分析。
- QUANTITATIVE COMPARISON OF DIFFERENT LYMPHOID CELLS FOR BAM HI W FRAGMENT OF EPSTEIN-BARR VIRAL NUCLEIC ACID Epstein-Barr病毒DNA Bam HI W片段在不同淋巴细胞株中量的比较
- 5.Methods By RT PCR DDH,RT nested PCR,RNA DDH,we detect the nucleic acid of HCV and deduce the specificity,sensitivity and stability of RT PCR DDH in viral nucleic acid detection. 核酸与各类物质的相互作用与探测核酸的结构与功能密切相关是揭示核酸的生物功能和一些药物的作用机制的重要途径。