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- Cell density was associated with PEI/DNA transfection efficiency. 细胞密度影响转染效率;
- The main problem of virus and nonvirus vector is safety issue and low transfection efficiency. 病毒类载体和非病毒类载体最大的不足之处在于不安全和转染效率低。
- The transfection efficiency was influenced by different incubate time between ESCORT-DNA mixture and lung cancer cells. 脂质体ESCORT-DNA混合物与细胞孵育时间不同对转染效率也有一定的影响。
- Additionally, PEI is considered to exhibit high transfection efficiency and endosomal disrupting capacity. 此外， PEI也被认为具有高转染率和使内涵体破裂的能力。
- AIM To investigate the factors affecting transfection efficiency of antisense oligodeoxynucleotides (ASON) by cationic fusogenic liposome (CFL). 目的研究阳离子膜融合脂质体(CFL)介导反义寡核苷酸(ASON)的细胞转染效率及影响因素。
- Effects of Incorporation of Poly( g -Glutamic Acid) in Chitosan/DNA Complex Nanoparticles on Cellular Uptake and Transfection Efficiency. 此篇论文曾荣获 2008 年高分子联合会议壁报论文竞赛第一名。
- The enhanced green fluorescent protein(EGFP) plasmid was used as positive control and the transfection efficiency was detected by flow cytometry. 同时转染带有增强型绿色荧光蛋白(EGFP)的载体作为阳性对照,流式细胞术检测其绿色荧光以确定转染效率;
- The transfection efficiency of rAAV1/2-lacZ vector gene from umbilical mesenchymal stem cells cultured in vitro is satisfactory and becomes higher as MOI increased. 体外培养的脐血间质干细胞能高效转染重组腺相关病毒1/2载体-lacZ报告基因,在一定感染复数范围内,细胞转染率随着感染复数的增加而升高。
- This study was aimed to investigate the transfection efficiency of adenoviral vector AD5/F35 to hematopoietic malignant cells lines of various origins and AD5/F35 cytotoxicity. 摘要本研究比较AD5/F35腺病毒载体对不同来源的血液系统恶性细胞系感染效率的差异和细胞毒性反应。
- Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy. 摘要腺病毒载体具有高效转染和低细胞毒性的特点，已成为基因治疗研究中应用最广泛的载体之一。
- Larger (200-250 nm) and smaller (50 nm) nanoparticles were compared to evaluate the effects of size on transfection efficiency as well as any associated intraarticular reaction. 文中比较了大（200-250nm）、小(50 nm)颗粒的结果，希望以此了解尺寸效应对转染率及其它相关的关节内反应的影响。
- The effect of 12C6+ beam irradiation on AdCMV-GFP(a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. 探讨12C6+离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒(AdCMV-GFP)转染小鼠黑色素瘤细胞(B16细胞系)的影响。
- Adenovirus as gene transfer vector is characterized by high transfection efficiency,high titer,good stability in target cells and low pathogenicity,and has been widely used in gene therapy. 腺病毒载体是一种非常有效的转基因载体,具有高效率、低致病性、高滴度及不整合入宿主细胞染色体等优点,广泛应用于基因治疗。
- The study observed the transfection efficiency of recombinant adeno-associated virus with enhanced green fluorescent protein transduction to rat bone marrow mesenchymal stem cells in vitro. 如果进一步在基因水平上分析转染后的骨髓间充质干细胞有哪些生物学特性，如外源基因的表达、细胞凋亡的变化等，实验结果会更加完善和系统。
- By selecting the proper amphiphilic polymer, stable PEGylated polyplexes were fabricated successfully to improve stability and transfection efficiency at a physiological salt concentration. 通过对两亲聚合物中疏水链段的选择调控，可制备稳定的PEG化基因超分子组装体，提高基因传递体系在生理盐溶液中的稳定性及基因转染效率。
- Adenovirus as gene transfer vector is characterized by high transfection efficiency, high titer, good stability in target cells and low pathogenicity, and has been widely used in gene therapy. 摘要腺病毒载体是一种非常有效的转基因载体，具有高效率、低致病性、高滴度及不整合入宿主细胞染色体等优点，广泛应用于基因治疗。
- FnCBD_(64) transfection efficiently promote adhesion of EPCs on decellularized xenograft aortic valves. Still, much work is needed in the future study of TEHV. 携带信号肽序列的Ad.;FnCBD_(64)转染EPCs可有效提高细胞的粘附力;有助于TEHV的构建;但目前方法构建的TEHV还不能耐受大流量流体的冲刷;未来TEHV的研究还有很多问题需要解决。
- When the liver cells were transfected with pEGFP-N3TPT1,the bright green florescence in transfected cells was observed. Transfection efficiency was about 30%. 用此重构体转染后,在荧光显微镜下,可见细胞发出明亮的绿色荧光,转染效率约在30%25左右。
- We must improve our efficiency as soon as possible. 我们必须尽快地提高工作效率。
- MSCs were transfected with enhanced green fluorescent protein (EGFP) gene under the mediation of LipofectAMINE, and G418 resistance was adopted for screen, so as to observe the transfection efficiency. 在脂质体介导下,将增强的绿色荧光蛋白基因导入骨髓间充质干细胞,然后通过G418抗性筛选,观察转染效率。
