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- METHODS: The eukaryotic expression vector pcDNA3.0 containing human full length HAb18G cDNA and empty vector pcDNA3.0 were introduced respectively into NIH/3T3 fibroblast cells by liposome mediated gene transfection method. 方法 :将含有肝癌相关抗原HAb1 8G全长cDNA的真核表达载体 pcDNA3.;0 /HAb1 8G利用脂质体介导法转染小鼠成纤维细胞NIH/ 3T3;G4 1 8筛选建立稳定表达阳性克隆细胞株t3T3; 利用流式细胞仪、间接免疫荧光染色方法鉴定目的蛋白转染情况 ;
- AIM: To investigate an effective transfection method of non-viral vectors and the expression of enhanced green fluorescent protein (EGFP) in the transfected immortalized neural progenitor cells (INPC). 目的:寻找能高效转染永生化神经前体细胞(IN-PC)的非病毒载体;观察转染后增强型绿色荧光蛋白(EGFP)的表达.
- Abstract： Objective： The aim of this study was to investigate vitro/vivo killing effect of suicide gene CD on the human gastric cancer cell line 7901 by gene transfection method. 摘 要： 目的：通过转基因方法观察自杀基因CD对人胃癌细胞株7901的体内外杀伤作用，并观察组织病理变化。
- Their business methods sicken me. 我很讨厌他们做生意的手法。
- There are no infallible methods. 没有绝对可靠的方法。
- The old methods proved best after all. 采用老方法结果反而是某事物最好。
- She's not too nice in her business methods. 她的经营方法不敢恭维。
- Employment of empirical methods, as in science. 经验方法的运用，例如在科学中
- We'll try to improve our teaching methods. 我们要设法改进教学方法。
- These methods have fallen into disuse. 这些方法已废而不用了。
- The two methods were in effect identical. 那两个办法实际上是一样的。
- Geometrical theorems grew out of empirical methods. 几何定理是从经验得出的。
- transfect methods 转染方法
- One who makes a living by dishonest methods. 骗子通过非诚实的方法谋生的人
- The methods used have helped enormously in cutting out deadwood. 这些方法在废物利用方面起了相当大的作用。
- Which of the two methods is superior? 这两种方法哪一种好?
- He worked out methods to destroy bacteria on the seeds. 他研究出消灭种子上细菌的方法。
- The new system is a departure from our usual methods. 这套新办法不同于我们惯用的方法。
- Conservation methods will also help meet their energy needs. 能量保存方法也会有利于满足其能源需求。
- People have found many humane methods of killing animals. 人们已经发明了很多宰杀动物的人道方法。
