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thymidine kinase minus mutant 胸苷激酶阴性突变株
Objective Preparition of monoclone antibody to human thymidine kinase(hTK). 目的制备人胸苷激酶单克隆抗体。
Objective To evaluate the bystander effect in the retrovirus vector the herpes simplex virus thymidine kinase gene Ganciclovir system (RV HSV tk GCV). 目的研究反转录病毒载体 (RV) -单纯疱疹病毒胸苷激酶基因 (HSV tK) -环丙氧苷 (GCV)系统中的旁观者效应。
We have identified conserved domains of the thymidine kinase proteins of the alpha herpes viruses:-R*Y*DG**G*GK*T-and-FDRHP*A***C*P*AR-. 在氨基酸序列中发现有疱疹病毒TK基因的保守序列R*Y*DG**G*GK*T-和-FDRHP*A***C*P*AR-。
ILTV has thymidine kinase ( TK ) gene which has been shown is associated with its virulence and reactivation from the latent state. TK ) 病毒毒力基因，此基因与病毒毒力及病毒于潜伏期再活化的能力有关。
Objective To study the mechanism of bystander effect in herpes simplex virus thymidine kinase(HSV TK)/Ganciclovir(GCV) mediated gene therapy. 目的探讨用单纯疱疹病毒胸腺嘧啶核苷激酶（HSV?TK）/丙氧鸟苷（GCV）系统进行基因治疗时发生的旁观者效应机制。
Next, patients are given an antiviral drug, Ganciclovir, which the thymidine kinase then converts into a substance that destroys all dividing cells. 接下来，将抗病毒药物Ganciclovir注射到病人体内，Ganciclovir能将胸苷激酶转化为一种能杀死所有分裂细胞的物质。
Objective: To observe the killing effect of Herpes Simplex Virus thymidine kinase gene/Acyclovir system(HSV tk/ACV) on human hepatocarcinoma cells in vitro. 目的 :观察单纯疱疹病毒胸苷激酶基因 (HSV - tk基因 ,自杀基因 )与阿昔洛韦 (ACV)系统对人肝癌细胞的体外杀伤效果。
Objective:Amplification, cloning, and expression of 3 intact gene fragments encoding for DNase, thymidine kinase and BHRF1 protein in Epstein?Barr virus (EBV). 目的：扩增克隆及表达EB病毒（EBV）3种（DNA酶、胸苷激酶及BHRF1）全基因片段。
Another study employed the AFP gene promoter for gene therapy against high AFP-secreting hepatoma cells using a retrovirus vector carrying a herpes simplex thymidine kinase gene . 另一研究通过携带单纯性疱疹病毒胸腺嘧啶激酶基因（的逆转录病毒载体，使用AFP基因启动子基因治疗高AFP分泌肝细胞瘤。
Another study employed the AFP gene promoter for gene therapy against high AFP-secreting hepatoma cells using a retroirus ector carrying a herpes simplex thymidine kinase gene . 另一研究通过携带单纯性疱疹病毒胸腺嘧啶激酶基因（的逆转录病毒载体，使用AFP基因启动子基因治疗高AFP分泌肝细胞瘤。
Objective To evaluate the efficacy of gene therapy with herpes simplex virus thymidine kinase (HSV - tk) gene transfer followed by ganciclovir (GCV) therapy in lung adenocarcinoma. 目的探讨单纯疱疹病毒胸苷激酶(HSV-tk)基因转染联合抗病毒药物更昔洛韦(GCV)对人肺腺癌细胞系的杀伤作用。
Objective To investigate the augmentation effect and mechanism of all trans retinoic acid(ATRA)on the bystander effect of herpes simplex virus thymidine kinase (HSV TK)/ganciclovir(GCV) system in the treatment of tongue carcinoma cell line. 目的探讨全反式维甲酸 (all transretinoicacid ,ATRA)对单纯疱疹病毒胸苷酸激酶(herpessimplexvirusthymidinekinase ,HSV TK) /丙氧鸟苷 (ganciclovir,GCV)系统治疗舌鳞癌旁观者效应的增强作用及机制。
Objective: To evaluate the efficacy of gene therapy with the herpes simplex virus thymidine kinase (HSV TK) gene transference followed by ganciclovir (GCV) therapy in ovarian cancer. 目的：探讨单纯疱疹病毒胸苷激酶（HSV－TK）基因转染联合抗病毒药物羟基无环鸟苷（GCV）对人卵巢癌细胞系的杀伤效应。
Objective:To evaluate the treatment effect of retrovirus mediated herpes simplex virus thymidine kinase(HSV 1 tk) gene transference followed by administration of ganciclovir(GCV) and acyclovir(ACV) on ovarian epithelial cancer cells. 目的 :探讨逆转录病毒载体介导的单纯疱疹病毒胸苷激酶 (HSV1 tk)基因转染 ,联合抗病毒药物羟甲基无环鸟苷 ( ganciclovir ,GCV)和无环鸟苷 (acyclovir ,ACV )对人卵巢上皮癌 (OEC)细胞系TYK的杀伤作用。
A thymidine kinase(TK) gene insertion-deleted strain of avian infectious laryngo-tracheitis virus (ILTV), the first genetically engineered vaccine strain candidate for ILT control in our country was developed at our university. 鸡传染性喉气管炎病毒(ILTV)胸苷激酶(TK)基因插入缺失毒株是由我校研制的国内首次构建的ILT基因工程疫苗候选株。
A transfering vector was constructed by inserted NDV F gene and IBDV VPO gene, under the control of two synthetic promoters ATI P7. 5X20 which were conjugated at opposite direction inserted within the thymidine kinase (TK) gene of FPV 282E4 strain. 将NDV F基因和IBDV VPO基因分别置于人工合成的复合启动子ATI·p7.;5×20的下游，将两个表达单元以复合启动子反向方式连接，插入TK基因中，得到两个复合启动子分别调控两个不同基因的重组转移质粒pVPO-2ATI·p7
TK-gene system (retrovirus transfer of the herpes simplex virus thymidine kinase gene) is one of the much more mature plans. We have succeeded in transmitting herpes TK gene into human pancreatic cancer cells, named SW1990 by retrovirus. 由缺陷型逆转录病毒介导的TK基因系统（RV－HSV－TK）是众多肿瘤生物治疗方法中一个技术较为成熟的方案，本实验采用该系统在体外成功地转染了人胰腺癌细胞株SW1990并能够稳定传代培养，转染了TK基因的SW1990细胞（SW±K），其生长曲线与未转染的SW1990细胞无差异。
It indicated that toxin minus mutants of E.chrysanthemi pv. zeae could reproduce in the rice, but they couldn’t cause symption. 说明，毒素突变体能在水稻体内定殖，但不引起水稻发病，表明毒素在病菌致病性中作用重要。
The target gene C-erbB-2 and control gene thymidine kinase are located on chromosome 17. The different size of two PCR fragments were produced by simulataneously performing PCR on test and control genes in the same PCR mix. 靶基因C-erbB-2和对照基因thymidine kinase均定位于17号染色体。在同一反应体系中进行PCR反应可产生两个大小不同的PCR扩增的特异性片段，然后通过2%25琼脂糖凝胶电泳将两片段分开，再用密度测定仪测定靶基因与对照基因的密度比率，当两者密度比率大于1时，表明存在C-erbB-2原癌基因扩增。