the cgh gene was cloned into baculovirus transfer vector pBacPAK-His, and homology recombinant with linear baculovirus Bm-BacPAK-6 in Bombyx mori BmN cell, the recombinant viruses were screened by plaque assay.

• 将cgh基因克隆进杆状病毒转移载体pBacPAK-His,在家蚕BmN细胞中,与线性化杆状病毒Bm-BacPAK-6重组,通过空斑筛选,获得重组病毒。