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- hepatocyte - targeted gene transfer 肝定向基因转移
- targeted gene transfer 定向基因转移
- Viruses can be used as gene transfer vehicles. 细菌可用作基因转移的媒介。
- In gene therapy, gene transfer is targeted into specific tumor tissue by using relatively specific markers in brain gliomas such as EGFR, telomere and telomerase. 在胶质瘤的基因治疗中,利用脑胶质瘤组织中相对特异性的标志物如表皮生长因子受体、端粒及端粒酶等实现目的基因在肿瘤组织的靶向性表达;
- Somatic gene transfer only targets body cells (somatic cells). 体细胞基因只能被转进体细胞。
- KSC have become ideal research target cells in gene transfer, since abnormal epidermal gene expression can be regulated through stem cell gene transfer. KSC已成为转基因理想的靶细胞，可通过干细胞的基因转移来调节表皮异常基因表型；
- Liver Stem Cells as Vector for Targeted Gene Therapy of Hepatocellular Carcinoma. 肝干细胞作为肝细胞癌靶向基因治疗载体的实验研究
- Analyse the genotype of the target genes using PCR. 聚合酶链反应(PCR)扩增目的基因进行基因型分析。
- Targeted gene replacement (TGR) is an important technique for gene function analysis. 目标基因替换是基因功能分析的重要手段。
- As a mammalian gene transfer vector, baculovirus owns some unique advantages. 杆状病毒作为哺乳动物细胞基因转移的载体有很多独特的优点。
- Horizontal gene transfer (HGT) is a cross-species form of genetic transfer. 横向基因迁移是指跨物种形式的遗传迁移。
- AIM: To clone human soluble TRAIL cDNA and construct its eukaryotic expression vector to prepare a condition for targeted gene therapy. 克隆人可溶性trail(strail)基因并构建肿瘤细胞特异性真核表达载体;为肿瘤基因治疗的靶向性奠定实验基础.
- Molecular marker-assisted selection (MAS) provides a very efficient tool for backcross breeding because it can offer rapid and precise selection of the targeted gene. 分子标记辅助选择技术以其对目标基因快速而精确的选择为回交育种提供了非常有效的工具。
- To determine the target cell types and the transduction efficiency of AAV vector-mediated gene transfer, the expression of TH, AADC or was assayed by using immunohistochemical staining. 采用免疫组化方法确定TH、AADC和GCH在纹状体内的表达。
- The silencing of target gene transcription by unliganded NRs(including AR) may be mediated by these corepressors. 非配体化的核受体(NRs)抑制靶基因的转录可能是通过这些辅阻遏子来介导的。
- Effect of eNOS Gene Transfer on the Capacity of Hepatic Sinusoids in cirrhotic rats. eNOS基因治疗对肝硬化大鼠肝窦容积的影响
- Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR. 应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
- Latter, we observed the effects of ATF gene transfer on invasive potential of cancer cells in vitro. 观察ATF基因转移对肿瘤细胞体外侵袭人工基底膜能力的影响;
- The target gene was then inserted into expression vector pET-28a and expressed in E.coli BL21 with IPTG induction. 将mdlA基因克隆到原核表达载体pET-28a中，转化大肠杆菌BL21(DE3)，经诱导后，重组的脂肪酶在宿主菌中的表达量可达菌体总蛋白量的45.;2%25。
- Copolymer PEG-PEI(2-25-1) was proved to be a better carrier for in vitro gene transfer. 共聚物PEG-PEI(2-25-1)被证实为较有效的介导体外基因传递的复合物。
