您要查找的是不是：
- Targeted gene replacement (TGR) is an important technique for gene function analysis. 目标基因替换是基因功能分析的重要手段。
- Abstract: To improve the efficiency of targeted gene replacement (TGR), a dual screen (DS) system with gusA gene as negative selective marker (GUS-DS) was developed in Magnaporthe oryzae. 摘 要: 目标基因替换是基因功能研究的重要方法, 在生物工程领域广泛应用。
- targeted gene replacement 定向基因置换, 靶向基因置换
- Liver Stem Cells as Vector for Targeted Gene Therapy of Hepatocellular Carcinoma. 肝干细胞作为肝细胞癌靶向基因治疗载体的实验研究
- AIM: To clone human soluble TRAIL cDNA and construct its eukaryotic expression vector to prepare a condition for targeted gene therapy. 克隆人可溶性trail(strail)基因并构建肿瘤细胞特异性真核表达载体;为肿瘤基因治疗的靶向性奠定实验基础.
- Analyse the genotype of the target genes using PCR. 聚合酶链反应(PCR)扩增目的基因进行基因型分析。
- Molecular marker-assisted selection (MAS) provides a very efficient tool for backcross breeding because it can offer rapid and precise selection of the targeted gene. 分子标记辅助选择技术以其对目标基因快速而精确的选择为回交育种提供了非常有效的工具。
- Gene therapy is a synthetically technique of physics, chemistry, biology, and medical science.It is applied in gene replacement, gene correction, gene augmentation, gene inactivation. 摘要基因治疗综合了物理、化学、生物、医学等多门学科的技术，应用于基因的置换、修正、修饰、失活。
- The silencing of target gene transcription by unliganded NRs(including AR) may be mediated by these corepressors. 非配体化的核受体(NRs)抑制靶基因的转录可能是通过这些辅阻遏子来介导的。
- Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR. 应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的。
- The target gene was then inserted into expression vector pET-28a and expressed in E.coli BL21 with IPTG induction. 将mdlA基因克隆到原核表达载体pET-28a中，转化大肠杆菌BL21(DE3)，经诱导后，重组的脂肪酶在宿主菌中的表达量可达菌体总蛋白量的45.;2%25。
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
- The therapy of antiangiogenesis and target gene offer a broad clinic prospect for lung cancer. 内皮抑素的抗血管生成治疗和血管靶向基因治疗,为治疗肺癌提供了广阔前景。
- Target gene combined with proper vector is transformed in Ecoli cells to amplify. 用适当的载体与目的分子连接后，转入大肠肝菌宿主细胞扩增。
- It was reported in the past decade that a targeted gene inactivation of some transcription factors could specifically affect the development of some groups of neurons in the brainstem and then resulted in distinct respiratory phenotypes. 近年来，人们利用分子生物学技术研究发现，小鼠某些基因突变可影响呼吸中枢特定神经元的发育，并由此导致特殊的呼吸表型。
- A previous study has mapped the Ihd gene onto chromosome 10 with SSR markers, but the linked markers were found on one side of the target gene. 先前的研究利用SSR标记已将lhd基因定位在第10染色体上，但只在目标基因一侧找到标记。
- The progress in establishment of regeneration system of Brassica campestris and its ge netic transformation method,influence factor,screening marker and targeted gene were reviewed in this paper. 对芸薹属植物再生系统建立方面所取得的进展,从转化方法、影响转化的因素、筛选标记及目的基因的研究等方面对芸薹属植物外源基因的遗传转化的研究情况进行了综述。
- RESULTS: The target gene of recombinant adenovirus plasmid could be amplified by PCR in different passages of HEK293 cells. 用减蛋综合征阳性血清在体外中和重组腺病毒，接种HEK293细胞；
- METHODS: The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. 方法 :从正常前列腺组织中钓取PSP94cDNA ,构建重组质粒pUC19-PSP94 ,转化后提取目的基因并进行测序。
- The sequence of target gene of recombinant plasmid pcDNA3-ALR constructed in this experiment was in accordance with the sequence of rat ALR cDNA reported. 构建的pcDNA3-ALR重组质粒目的基因与文献报道的大鼠ALR cDNA序列相同。
