tail flick test(TFT) was used to test rat algesthesia,and stopwatch was used to record the time for tail flick(s) that was taken as the value of pain threshold.

• 采用辐射热甩尾法测定大鼠痛觉,用秒表测定大鼠甩尾反应时间(s)并作为痛阈值。