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- Then the subtracted library was screened by PCR and the differential fragments were sequenced and analyzed with BLAST. 对差减文库进行PCR筛选,对得到的差异片段进行测序及BLAST分析;
- Objective:To identify the differentially expression of the gene perforin from the subtracted libraries between asymptomatic HBV carriers(ASCs) and the normal control subjects. 目的:验证无症状乙型肝炎病毒携带者(A SC s)与正常对照组消减文库中的差异基因穿孔素(perforin)在相应人群中的差异表达。
- Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA. 目的:消减文库构建过程中,用PCR技术快速筛选重组阳性克隆。
- Recombinant clone can be analyzed directly with the use of PCR.It is very efficient,especially in establishment of subtractive library of cDNA. 筛选重组阳性克隆可直接用细菌悬液作PCR模板,在消减文库构建时,能大大提高工作效率。
- The methods in cDNA subtractive library construcdon have been reported a lot, and also developed two kinds of directions , btt no one is perfect still now. 近年来报道了多种构建方法,并形成了两个发展趋势,但目前还无一种方法被普遍认可。
- If you subtract 2 from 8, you get 6. 8减去2是6。
- The college library is a favorite haunt. 大学图书馆是人们喜欢的地方。
- He is a vast library in epitome. 他脑中有万卷藏书。
- Subtract 3 from 7 and you have 4. 七减三得四。
- He gave away his books to a circulating library. 他把自己的书籍损赠给一家循环图书馆。
- The library boasts first edition of Shakespeare. 这个图书馆以藏有初版的莎士比亚集而自豪。
- I have the book on loan from the library. 我从图书馆借来这本书。
- I passed the store on my way to the library. 我在去图书馆的路上经过了那家商店。
- His hunger for knowledge drove him to the library. 他对求知的强烈渴望驱使他上图书馆。
- The library was enriched with new books. 购买新书使图书馆得到了充实。
- Students must have access to a good library. 学生要有使用好图书馆的便利条件。
- An amount or quantity added or subtracted in order to correct. 改正量为改正而加入或抽除的数或质
- You can look for the book in the library catalog. 你可以在图书馆的图书目录上查找这本书。
- The little girl has begun to learn to add and subtract. 这小女孩已在学做加减法了。
- He find what he want by grub around in the library. 他在图书馆乱翻一通终於找到了需要的资料。