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- Method: (1) NS3 protease gene and single chain NS3/4A gene were both subcloned into pQE-30 and expressed in E. 方法:(1) 用原核表达载体pQE-30分别构建含有NS3蛋白酶基因的pQE/NS3和含有单链NS3/4A基因的pQE/NS3/4A重组质粒;
- The isolated plasmid PMPR4. pMPR8 pMPR16. harboring the neutral protease gene were used to transform four B. subtilis strains ASl. 398, ML,DB104 and BG 2036. The protease activity of transformatnts showed a significant increase. 用含有中性蛋白酶基因的重组质粒pMPR4、pMPR8和pMPR16转化BacillussubtilisBG2036，BacillussubtilisAS1.;398和BacillussubtilisML，对转化菌产生的蛋白酶活性分析表明：三个重组质粒的导入，均使宿主菌的酶活性有大幅度的提高。
- Altogether 19 strains belong to 13 species of ribosomal protein mutants of Bacillus subtilit were tested in vitro transcription-translation system for their influence on the translation of alkaline protease gene (apr). 用枯草杆菌体外转录-翻译偶联系统检测13种19株枯草杆菌核糖体蛋白质突变对碱性蛋白酶基因表达的影响,发现10种13株核糖体蛋白质突变能影响碱性蛋白酶基因的表达。
- Aspergillus oryzae alkaline protease gene 米曲霉碱性蛋白酶基因
- You can keep my book as long as you like. 我的书你要借多久都随你的便。
- I would like to have a word with you. 我想同你说句话。
- Expression of Cysteine Protease Gene in Bm-BmNPV Expression System 半胱氨酸蛋白酶基因在家蚕杆状病毒表达系统中的表达
- An idea popped into his mind like a flash. 他头脑里突然闪过一个念头。
- Sduty On The Expression Of Neutral Protease Gene from Bacillus Subtill 枯草杆菌中性蛋白酶基因的表达
- Some young people like to make a fetish of style. 有些年轻人喜欢盲目地赶时髦。
- She was like a narcissus trembling in the wind. 她像一株在风中摇动的水仙。
- The driven gear fits its driving gear like a glove. 这个从动齿轮与它的主动轮啮合得非常好。
- A man of great ability like him will eventually reach the top. 像他这样才能出众的人终究会升到最上层的。
- He talks like a parrot and just repeats what he heard. 他只是鹦鹉学舌，拾人牙慧罢了。
- After a week's work I like to retreat to the country to relax. 工作一周之後，我喜欢躲到乡下松弛一下。
- I must say I didn't like the cut of his jib. 说实在的，我不喜欢他那个样子。
- That girl over there looks like she's in dreamland. 那边的那个女孩看上去像在梦境里一样。
- The dog clung like a limpet to the thief. 那条狗死死缠住小偷不放。
- Don't speak like that. It sounds insincere to me. 别这样讲话，这样我听起来感到不诚恳。
- I like to curl up with a story book. 我喜欢蜷曲而卧着看故事书。
