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Stable expression of AIRE was confirmed by fluorescence microscope, FACS and western blotting analysis. 经荧光显微镜、流式细胞仪及免疫印迹法检测 ,证实 AIRE基因得到了稳定表达。
Transgenic plants with high and stable expression are ideal to get by plant gene engineering, but gene silencing limits their application. 摘要植物基因工程研究是希望获得高稳定表达的转基因植株，而转基因沉默现象却限制了转基因植物的应用前景。
AIM: To establish a rat myoblast line (L6) with stable expression of human calcitonin (hCT) gene and observe the hCT expression and secretion in L6 cells. 目的:建立稳定表达人降钙素(hCT)基因的细胞株(L6),观察人降钙素基因在大鼠成肌细胞中的表达和分泌情况。
Results (1) 5 stable expressing hTM clonal cells lines were obtained. Western blotting分析显示McAb NH-1可特异地识别还原条件下相对分子量为105KD左右的蛋白多肽。
Two recombinant human granulocyte colonystimulating factor expression plasmids pED-GCSF and pEF-GCSF were constructed. Each plasmid was transfected into the COS7 cells for transient expression,and into CHO-dhfr - cells for stable expression. 利用PCR反应、DNA测序、基因重组等技术，构建了两个表达人粒细胞集落刺激因子cDNA的重组质粒pED－GCSF和pEF－GCSF，两质粒分别转染COS7细胞作瞬时表达，转染CHO－dhfr－细胞作稳定表达。
IL-29 was cloned and stably expressed in cos-7 cells successfully. 成功获得人IL-29全长cDNA并在cos-7细胞中稳定表达。
These expression plasmids were transfected into HepG2-N10 cells, a cell line which stably express HBsAg,HBeAg and adw2 subtype Dane Particles. HepG2-N10是一个可稳定表达HBsAg及HBeAg的细胞株(其表达HBV血清型为adw2亚型)。
The evils ensue from lack of a stable government. 这些弊病是由于缺乏一个稳定的政府而引起的。
Stable Expression of Anti-HPV16 E7-ribozyme in CV-1 Cell Line 抗HPV16 E7-ribozyme在CV-1细胞中的稳定表达
Keywords COS7 cell;stable expression;SV40 vector;episomal DNA; 关键词COS7细胞;稳定表达;SV40表达载体;附加体DNA;
The horse in the stable has broken out. 马厩里的那匹马跑了。
An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
He always shows a doleful expression. 他总是露出忧郁的表情。
He is a man of stable character. 他是一个稳重可靠的人。
The black horse is one of Mr. King's stable. 那匹黑马是金先生的赛马之一。
That country is politically stable. 该国政治上稳定。
I can think of no greater expression of defeatism. 这是我听到的最悲观的失败主义论调了。
He has a whole stable of informers working for him. 他有一群告密者为他效劳。
A constant, variable, function name, or expression. 一种常数、变量、函数名或表达式。
There were six stalls for horses in the stable. 马厩里有六个栏。