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- Objective: To explore the effects of cryopreservation on sperm DNA integrity of infertile men. 摘要目的探讨冷冻保存对不育患者精子核DNA链完整性的影响。
- Method: Sperm DNA integrity of 40 semen samples from nonoligospermatism infertile men were detected by the using of comet assay before freezing and after thawing. 方法采用彗星试验检测40例非少精子症不育患者精液冷冻前及复苏后精子核DNA链的完整性。
- sperm DNA integrity 精子DNA完整性
- Cryopreservation of human spermatozoa and sperm preparation techniques all influence the integrity of sperm DNA. 同时,精子低温冷冻保存和诸多体外处理技术均能影响精子DNA的完整性。
- The DNA integrity of sperm is essential for fertilization,as well as for the normal development of the blastocyst,embryo and child. 精子DNA完整性异常,会影响受精和胚胎发育,最终导致不孕不育、流产、死产、子代智力低下和染色体疾病。
- Conclusion: Cryopreservation does not affect DNA integrity of undamaged sperm of infertile men, however, it can increase the degree of DNA damage of sperm that had been damaged before freezing. 结论精液冷冻对不育患者完整的精子核DNA链无影响,但可能加重冷冻前已有损伤精子核DNA链的损伤程度。
- Study on Interaction between Hematoporphyrin Dihydrochloride and Herring Sperm DNA by Spectroscopy[J]. 引用该论文 Li Hongbo;Ta Honggui;Wang Xingming;Wang Suqin;Ding Lisheng.
- Raman spectra of herring sperm DNA in aqueous solution and after UVA &UVB ultraviolet radiation by 0. 本文检测了鲱鱼精DNA水溶液和经UVA和UVB紫外线辐射后的拉曼光谱。
- There is increasing evidence that sperm DNA anomalies may lead to infertility,abortion,stillbirth,fathead and chromosomal diseases. 传统的精液检查往往不能找到精子DNA完整性异常患者的不育原因,因此对这部分患者进行精子DNA完整性检查具有更重要的意义。
- The interaction of metal complex of norfloxacin(NFLX) with herring sperm DNA and its characteristic were studied by UV and visible spectrophotometry. 用紫外可见分光光度法研究了诺氟沙星(NFLX)的光谱特性及其金属络合物与鲱鱼精DNA的相互作用。
- Oxidative stress,deficiencies in natural processes such as chromatin packaging and abortive apoptosis are the main factors that lead to sperm DNA damage. 氧化胁迫、精子染色质包装或分离异常、凋亡异常发生等因素可引起精子DNA损伤。
- He studied 118 men with above-average sperm DNA damage and found the quality of their sperm increased significantly after they were told to ejaculate daily for seven days. 格里宁对118位男性进行了研究,这些男性的精子DNA受损程度高于平均水平。研究结果发现,在要求被调查者连续七天射精之后,他们的精子质量显著改善。
- The modulation of CTAB on the thermal stability of salmon sperm DNA and herring sperm DNA have been investigated by employing differential scanning calorimetry and ultraviolet spectroscopy methods. 用差示扫描量热法和变温紫外光谱法研究了CTAB对鲑鱼精DNA及降解的鲱鱼精DNA热稳定性的影响,发现CTAB使鲑鱼精DNA的热稳定性降低,但降低程度随CTAB浓度的增大而先增大后减小;
- Methods: The sperm DNA were analyzed with the flow cytometer.It was from the quantity of haploid, diploid and polyploid of DNA to judge the quality of sperm and its improvement after treatment. 方法:用流式细胞仪分析患者精液DNA,从DNA的单倍体量、二倍体量及多倍体数量来判断精液的质量以及服五子衍宗丸合加味肾气丸后对精液质量的改善。
- HBV DNA integration might cause c-fos activation and/or p53 mutation, and then lead to the occurrence of liver cacinama. HBVDNA的整合可能引起c-fos的激活和/或p53的突变,从而导致肝癌的发生。
- and herring sperm DNA in synthetic samples. 第一部分 化学发光分析概述及抗坏血酸、DNA测定的研究进展。
- HBV need a viral-coded reverse transcriptase to replicate because the virion DNA is synthesized from an RNA template, and have their DNA integrated into cellular DNA during replication. 需要病毒编码的逆转录酶来增殖,因为病毒体DNA是从RNA模板合成的并在复制时将DNA整合到细胞DNA。
- The male sperm fertilizes the female egg. 雄性的精子使雌性的卵子受精。
- He puts a high value on integrity. 他对正直的评价很高。
- I'm rather doubtful about his integrity. 我对这事的忠诚颇有怀疑。