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- Southern blot technique developed to identify DNA fragments. 印迹杂交被用来发现DNA片段。
- southern blotting DNA 印迹法
- blot southern blot DNA 印迹
- The presence or absence of particular restriction sits can be ascertained using DNA probes in the technique called SOUTHERN BLOTTING. 不同个体限制酶的位点有很大的不同,因此可以被用来分析和比较基因组。
- Southern blotting analysis of genomic DNA indicated that enolase was likely to be a low-copy gene in the oilseed rape genome. Southern杂交结果显示烯醇酶以低拷贝形式在油菜基因组中存在.
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑点杂交法和 Southen 印迹法对脑瘤中 erbB、sis、myc 和 fos 这四种癌基因的扩增和重排进行了研究。
- Results of Southern blotting suggested that there were all a small gene family of MaMAPK, and MaMAPKgene may be small copies in genomic DNA of three species of Malus. Southern杂交结果表明,MoMAPK基因在3种苹果的基因组中均以低拷贝和基因家族形式存在,为进一步研究MaMAP基因的表达调控,揭示苹果属植物耐旱的分子机制奠定了基础。
- Total DNA of the 2 pathogens and their similar ones digested with EcoR I, were hybridized with mtDNA probes of 2ul8 and 4u40 by Southern blot, the RFLP patterns could identify F. 利用Southern杂交技术;将致病菌和近似菌的基因组DNA分别用EcoRI 酶切;与mtDNA探针2u18和4u40杂交的RFLP图谱可成功的鉴定F.
- With Southern Blotting hybridization of R plasmid DNA with gentamycin resistant gene probe from America, Australia and our lab,a different gentamycin resistant gene was found and thd new gene was cloned into pBluescript SK( I ) vector. 应用源自美国、澳洲及本室的庆大霉素耐药基因探针对分离的庆大霉素耐药菌株进行Southern印迹杂交,又发现了另一种与之不同源的庆大霉素耐药基因,并对该基因进行了分子克隆和初步分析。
- Methods: A quantitative analysis was carried out in mitochondrial DNA of peripheral blood cells from patients with mitochondrial myopathy/encephalomyopathy and normal controls by Southern blotting. 方法:用分子杂交技术对10例线粒体肌病/脑肌病患者和16例正常对照者的外周血细胞线粒体DNA进行定量分析。
- DNA dot blot analysis showed that 73. 4% of resistant plants contained GNA and hpt genes. Southern blot analysis confirmed the integration of GNA gene in the genome of transgenic rice. DNA点杂交检测表明73.;4%25的抗性植株同时含有GNA基因和hpt基因;Southern杂交分析进一步证实了GNA基因在转基因水稻基因组中的整合
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我们用常见的克隆载体pBR322作探针,用核酸点杂交和Southern吸印方法,检测了来自173人的213例样品。
- Southern Blot with positive plants of PCR showed that CMO and BADH have been integrated into the rice genome. 对随机抽取的PCR检测双阳性植株进行Southern blotting杂交检测分析,表明CMO和BADH基因均已整合到转基因水稻的基因组中。
- Southern blot analysis showed that Oncidium has at least 4 copies of EIN2 in its genome. 南方墨点分析显示EIN2至少有四个拷贝分布于基因体组内。
- Finally, on the basis of the established transformation protocol, rolB-pttGA20ox genes were stably transferred to the Populus alba by PCR and Southern blotting tests. 最后在此基础上,进行rolB-pttGA20ox双价基因的转化,并由PCR、Southern杂交和形态特征差异检测,以证明是否为转化植株。
- PCR assay and Southern blot analysis showed that CMO gene was integrated successfully into the genome of transformed plant. PCR及Southern检测证明CMO基因已整合进抗性植株基因组;
- With photosensitivebiotin lablled probes,HEV derived from clone ET1.1(418 bp)and ETV derived from Echo-13(500bp),Dot and Southern blotting were carried out on the NC membranes. 以光敏生物素标记HEVET1.;14186p探针,对硝酸纤维素膜进行点杂交和Southern印迹杂交。
- Transgenic plants were confirmed by PCR, PCR-Southern, and genomic Southern blotting which showed that the bar and pin II genes were co-integrated into the plant genome. 获得的转基因植株经PCR、PCR-Southern杂交以及植物基因组Southern杂交证实,目的基因已经整合入植物基因组中。
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA检测转基因马铃薯病毒抗性数据显示,温室条件下,3个转基因马铃薯株系(2M、3M、11M)具有病毒抗性,3个株系(1M、5M、10M)感病;
- Southern blot analysis and PCR of target segment in the genome of tobacco showed that all of the plants generated were the transgenic plants. 转基因植株总DNA PCR和Southern blot分析表明目的基因已经整合到植物基因组中,但转基因的拷贝数与抗病性无明显的相关性。