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- Leukemic colony formation unit (L-CFU) was cultured in soft agar medium in 10 cases with acute myeloblastic leukemia (AML) with PHA-LCM condition medium as a stimulating factor. 本文应用体外软琼脂培养技术,以PHA-LCM条件培养液作为白血病细胞集落形成刺激因素,对10例急性粒细胞白血病患者进行了L-CFU培养。
- soft agar medium 软琼脂培养基
- The colony forming efficincy in soft agar increased with the passaging. 软琼脂克隆形成率随着照后传代次数的增加而明显增高;
- The virulent strains had a polysaccharide capsule and formed smooth colonies on agar medium. 有毒菌株具有多糖荚膜它在琼脂培养基上形成光滑的菌落。
- The anchorage independent growth (soft agar colony formation) appeared in the 15th passage of BEAS-2B? NNK cells. 第15代BEAS2BNNK细胞具有锚着独立性生长特性(软琼脂克隆形成);
- The infected cells ( 293-BK cells ) grew as well as other 293 cells, and formed colony in soft agar 14 days later. 感染细胞(称作293-BK细胞)如其他293细胞一样生长;14天后可在软琼脂中形成细胞集落.
- Calli were produced from cotyledons of ramie variety Liuyang Daleyu on MSB agar medium containing 0. 5 mg/L 2, 4-D and 0. 5 mg/L KT. 苎麻品种浏阳大叶绿的子叶在含有2;4-D 0.;5mg/L、KT 0
- Current inspection of transformed cells by the method of double-major in soft agar cloning experiments and tumorigenicity in nude mice experiments. 目前检验转化细胞致瘤性的方法主要有双层软琼脂克隆实验和裸鼠致瘤实验。
- The result showed that adopting protein agar medium after pretreatment with SDS chemical method could significantly improve the benefit of isolating actinomyces from soil. 结果表明利用蛋白胨琼脂培养基结合SDS化学法样品预处理可以明显提高放线菌的检出机率;
- In this study, immortalized mesenchymal stem cell lines can be formed in soft agar cloning, cloning rate was only 2.1%, but not tumorigenicity in nude mice. 本实验的永生化间充质干细胞系可在软琼脂内形成克隆,克隆形成率只有2.;1%25,但不在裸鼠体内致瘤。
- Methods: The culture supernatant of cord blood LAK cells and diluted E.coli suspension were cocultured in agar medium and the colonies of E.coli were counted. 方法将脐血LAK细胞培养上清液和稀释的埃希氏大肠杆菌悬液混合,在培养皿内培养不同时间,计数各个培养皿内细菌菌落数。
- Compared with control cells, the result of soft agar culture showed that the cloning efficiency of SACC-83 cell, which is transfected with NCAM gene, decreased significantly. 4.;软琼脂培养结果显示,与对照组相比,转染了NCAM基因的SACC-83细胞克隆形成率明显降低。
- For further growth,the calli were transferred to 7 dif-ferent agar media,from which one suitable medium,MB_2,was selected. MB_2培养基(MB+甘氨酸2mg/l+NAA0.;2mg/l+BA2mg/l)对微愈伤组织增殖的效果最佳
- The positive clones were screened by G418.The proliferation,migration,and invasion of MDA-MB-231 cells before and after transfection were assessed by CFSE-flow cytometry,monolayer wound healing assay,and soft agar colony formation test. 应用CFSE-流式细胞仪检测,单层伤口愈合实验,软琼脂克隆形成实验等方法观察了MDA-MB-231细胞转染前后,细胞体外增殖及侵袭能力等指标的变化。
- The study aimed to establish a simple, inexpensive, nearly-maintenanceless and flexible hydroponic system for growing Arabidopsis thaliana plants by combining agar medium plus MS nutrients with the eppendorf tube system. 根据拟南芥的生长需求特点,采用MS培养基与营养液培养相结合的方法建立了一种简单、低耗、低维护和灵活的拟南芥植株水培系统。
- Two mutants,named mut-1 and mut-2,with decreased ADH activity were screened out by yeast peptone dextrose(YPD)agar medium containing allyl alcohol. These two mutants had decreased ADH activities of 41.63% and 50.29% compared with the parent strain. 在含丙烯醇的YPD筛选培养基上筛选获得两株ADH活力降低的突变株mut-1和mut-2;检测突变株mut-1和mut-2的最大ADH活力分别为35.;67和43
- Protoplast-derived calli were subcul-tured on the MSB agar medium with 0. 2 mg/L 2, 4-D and 0. 1 mg/L 6-BA and then transferred onto MSB medium containing 2. 0 mg/L 6-BA to induce shoots. 该愈伤组织在附加2;4-D 0.;2mg/L、6-BA 0
- B1 and B2 didn t secrete polysaccharose on beef polypeptone agar medium(BPA) and the nematode didn t grow on BPA medium with B1 and B2,while B1 and B2 secreted polysaccharose on PSA medium. 用牛肉膏蛋白胨细菌培养基培养B1和B2两种细菌时,细菌无多糖产生,线虫不能取食细菌而生长繁殖;当用马铃薯蔗糖真菌培养基培养B1和B2两种细菌时,细菌分泌丰富的多糖,线虫生长繁殖较好。
- Lots of mulberry-like gathered vesicles were found around and at the top of the hyphae cultured on agar medium under light microscope;expanded vesicles were occasionally found on hyphae. 于光显微镜下观察,发现洋菜培养基中之纯培养菌丝顶端及周围有许多团块聚集之桑椹状菌丝囊,菌丝上亦偶能发现膨大之囊状体存在。
- A rotary air sampler loaded with manital salt and Pseudomonas selection agar media strips were used to collect the samples from various sites. 使用装有甘露醇盐琼脂及绿脓杆菌特异琼脂之旋转式空气采样器进行采样。