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- Any single cell division in such a series. 细胞分裂这种一组分裂中的任何一次简单细胞分裂
- B7 1(CD80) cDNA was cloned by RT PCR from human B lymphma Raji cell line and confirmed by DNA sequencing. 应用RT-PCR技术从人B淋巴瘤细胞系Raji中克隆到B7-1(CD80)cDNA,并经测序证实。
- Methods: The expression of B7 in 22 cases of renal cell carcinoma was studied by RT PCR and immunochemical methods. 方法:本文应用RT?PCR和免疫组织化学技术检测了22例肾细胞癌组织B7的表达。
- Methods:Reverse transcription polymerase chain reaction (RT PCR) was used for determination of EMT expression in tumor cell lines. 方法:应用反转录多聚酶链反应(RT?PCR) 检测EMT 在肿瘤细胞系的表达。
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Genes can only be knocked out in a single cell. 基因的去除只有在单个细胞中才能进行。
- The intact cytoplasm of a single cell. 细胞质单细胞中未受损伤的细胞质
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- Element creates a single cell in a particular row. 元素在特定行中创建一个单元格。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- A single cell on a gravure cylinder. 中义照相凹版辊筒上的一个墨坑。
- Single cell 5-plex nest PCR for exons 17,19,44,45 and 48 of DMD gene was established. 获取正常男性单个淋巴细胞和无DMD家族史的单个胚胎细胞,行DMD基因外显子17、19、44、45、48的五重巢式PCR,分析扩增成功率、假阳性率和假阴性率。
- Expression of Fas was analyzed by a FACScan flow cytometry. Transcription and mutation of Fas gene were detected by RT PCR and single stranded conformation polymorphism(SSCP) analysis. 用流式细胞仪检测细胞表面Fas的表达 ,用RT PCR结合单链多态构象性分析 (SSCP) ,探讨Fas基因的转录及可能存在的Fas基因突变。
- Objective To explore the feasibility of using single cell fluorescent polymerase chain reaction(PCR)in the preimplantation genetic diagnosis(PGD). 目的建立单细胞水平的荧光PCR技术,探讨该技术对临床开展着床前遗传学诊断的可行性。
- Exect gene setuence hVEGF165 was obtained from the fetal human myocardium tissue by RT PCR; pcDNA3.1/VEGF165 was constructed ; and transient expression was going after transfecting COS 7 cell. RT PCR方法从胎儿心肌组织获得正确的hVEGF165基因序列,成功构建pcDNA3·1 /hVEGF165且实现转染COS 7细胞的瞬时表达。
- The theory that all living organisms are descended from a single cell or organism. 一元发生说认为所有生物都是由一个单细胞或有机体发展而来的理论
- This recombinant plasmid then was transfected into the eukaryotic cell COS 7, and the expression of LACK gene in eukaryotic cell was detected by RT PCR and immunofluorescent staining. 将此重组质粒转染 COS- 7细胞 ,通过 RT- PCR及免疫荧光检测 L ACK基因在真核细胞中的表达。
- Cotton fiber is a seed-hair consisting of a single cell. 棉花纤维是单一细胞的种子纤维。