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- In this study ,the PCR technique was developed for serotyping A.pleuropneumoniae using a set of specific primer designated for the apxI,apxII,apxIII and apxIV genes. APP 12种血清型分别具有不同的毒素基因（apxI,apxII,apxIII,apxIV）。
- After PCR anplification of total community DNA using a specific primer set,why is it usually necessary to eithercloneor run DGGE on the products before sequencing them? 相同的条带表示分子量相同但可能序列不同，需通过DGGE检测证明是一种DNA序列。
- The entire sequence of LT gene was amplified by PCR from Escherichia coli 216,using a specific primer based on the reported E. coli heat-labile enterotoxin gene sequence. 根据已报道的大肠杆菌不耐热肠毒素（LT）的基因序列设计引物，用PCR方法从Escherichia coli 216株基因组中扩增出LT基因的全序列。
- Objective To investigate the possibility of applying degenerate oligonucleotide primer PCR(DOP-PCR) and comparative genomic hybridization(CGH) in analysing genomic genetics of a single cell. 目的探索单细胞退变寡核甘酸引物PCR(DOP-PCR)-比较基因组杂交(CGH)技术应用于单细胞全基因组分析及着床前胚胎遗传学研究的可能性。
- Methods PCR-SSP was set up by synthesized 29 specific primers and 1 pairs of internal control primer,20 PCR reaction for DR alleles. 方法合成29个特异性引物和1对阳性对照引物,组成20个PCR反应用于DR位点,建立一步法PCR-SSP。
- Two pairs of specific primers were designed according to the conservative domain of PRV(gp50)and PPV(VP2). The zone of DNA amplification of single PCR appeared in 217 bp and 158 bp,respectively. 根据猪伪狂犬病病毒(PRV)和猪细小病毒(PPV)2种病原体的基因保守序列,分别设计并合成了与PRV的gp50基因序列和PPV的VP2基因互补的两对引物,进行单相PCR,分别扩增出预期的217bp片段及158bp片段;
- Objective: (1) To establish PCR reaction system that uses allele-specific primer PCR technique to detect SNP of KLOTHO gene. 目的:(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
- The personality was the prime reason in the single specific factors. 在单项具体因素中个人性格又是最重要的原因。
- The universal primer(CHS1 1S,CHS1 1R)is regarded as a specificity primer. The result showed that the sensitivity of universal primer PCR is 100 fg. 皮肤病原真菌通用引物(CHS1 1S,CHS1 1R)具有较强的特异性,敏感度为100 fg;
- Sequence specific primer ( PCR - SSP) 顺序特异性引物
- Sensitivity determination of universal primer PCR: Seven samples ranging from 100 ng to 100 fg shown positive,except the 10 fg sample yielding negative band. 皮肤病原真菌通用引物PCR敏感性测定,模板DNA 100 ng~100 fg的7个系列浓度均扩增出了阳性条带,10 fg为阴性。
- According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF). 根据GeneBank中TMV MP的序列设计基因特异引物，扩增出运动蛋白基因的整个开放阅读框（ORF）。
- Methods Type specific primers and PCR were used to detect the HBV genotypes of 127 Uighur CHB patients in Xinjiang. 方法采用型特异性引物巢式PCR法对127例维吾尔族慢性乙型肝炎患者进行基因分型,并测序验证。
- Barnard on long duration film exposures.The above image was taken in a single specific color emitted by hydrogen to bring out detail. 上面图像是在氢元素放射出单一特殊颜色下拍摄的，揭示了环的详细信息。
- A pair of specific primer was designed for amplification of cry2Ab gene. The amplified products of cry2Ab gene was directly inserted into pUCm-T vector and transformed into E. 根据已知的cry2Ab基因ORF序列，设计合成了一对特异性引物，PCR扩增出cry2Ab基因全长，并直接与pUCm-T载体相连。
- Methods HLA-B* gene polymorphism in 31 patients with DCM and 29 normal control subjects was analyzed using polymerase chain reaction-sequence specific primer(PCR-SSP) technique. 方法采用聚合酶链反应和顺序特异性引物(PCR-SSP)基因多态性分析方法,对31例扩张型心肌病患者及29例无血缘关系健康人的HLA-B*各等位基因及亚基因进行检测分析。
- Genomic DNA of the three yeast strains including TY-1, W1 and W2 were amplified by PCR with specific primers of delta sequence. 提取培养酵母TY-1和野生酵母W1,W2的基因组DNA,进行Delta-PCR,可以分别得到稳定而独特的DNA指纹图谱。
- Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba. 方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
- Primer pairs C1/C2, D1/D2 and G1/G2 amplified different fragments from several genomic DNA samples. Further study should be done to improved PCR conditions or search for other specific primers for the detection of Cur. 引物C1/C2、D1/D2和G1/G2的扩增结果：来自于Cur.
- A chitinase A gene (chiA) was successfully amplified by PCR using a pair of specific primers from Serratia marcescens Q901, and cloned into pUC19 vector. 用设计的特异性引物和PCR技术,成功地从粘质沙雷氏菌(Serratia marcescens)Q901基因组DNA中扩增并克隆几丁质酶A基因(chiA)序列(GenBank登录号:AY433954)。
- 今日热词
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries
