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- Objective To construct Sox9 siRNA expression vector in order to make clear the role of Sox9 in the genesis of chondrosarcoma. 目的构建Sox9siRNA表达载体,分析Sox9在软骨肉瘤中的作用机制。
- Construction of Tim-1 siRNA expression vectors and its inhibitory effect on Tim-IHA expressionFour pairs of oligonucleotide targeting to Tim-1were designed and synthesized. 针对Tim-1的siRNA表达载体的构建及其对Tim-1HA的抑制效果
- And LipofectamineTM 2000 mediated gene transfection of p498 siRNA expression vector into COS-7 cells downregulated the mRNA and protein level of 4-1BB at 48h after transfection. 结果经测序分析;各m4-1BB siRNA表达载体构建成功; 其中p498 siRNA与p4-1BB双质粒转染COS-7细胞;48h后可观察到细胞表面4-1BB分子明显下调;其胞内mRNA水平也显著降低;与无关干扰组比较;差异有统计学意义(P<0.;05)。
- HPV16 E7 specific siRNA expression vector can effectively inhibit the expression of E7 gene and partly reverse the malignant phenotype of CaSki cells,it may be one of the ideal strategies for gene therapy of cervical cancer. HPV16E7siRNA表达载体能有效地抑制宫颈癌CaSki细胞E7基因的表达,部分逆转其恶性表型。 因此它有望成为宫颈癌基因治疗的新策略之一。
- Construction of APRIL isoform-specific siRNA expression vector, screening by microfluidic chip electrophoresis and transfection optimization 表达载体构建、微流控芯片筛选及转染优化
- Construction of double siRNA expressing vector of Coxsackie virus B4 柯萨奇病毒B4双siRNA表达载体的构建及表达
- siRNA expression vectors 表达载体法
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题： 图1.;穿梭表达载体pRL_hEGF的构建。
- HLF;sperm;liposome;glandmammary special expression vectors;transgenic animals. 01人乳铁蛋白;精子;脂质体;乳腺特异表达载体;转基因动物
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- The sequences of RIP2 siRNA expressing plamid we had constructed was concordant with what we had designed. 2. 结果 1、构建的RIP2 siRNA表达质粒的序列与我们设计的一致。
- Cloning of ACC Oxidase Gene of Peach and Construction of Its Plant Recombinant Expression Vectors?? 桃ACC氧化酶基因的克隆和植物表达载体的构建
- Meanwhile, expression vectors were constructed with sence and antisence of PCaM-1 cDNA. 同时构建PCaM-1的正、反义真核表达载体，以转化花生研究CaM在胚胎发育中的作用。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。
