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- siRNA expression cassette 小干扰RNA表达框
- Construction and selection of siRNA expression cassettes targeting human telomerase reverse transcriptase gene in vitro 人端粒酶逆转录酶基因siRNA表达框架的构建和体外表达研究
- siRNA expression cassettes siRNA表达框架
- Objective To construct Sox9 siRNA expression vector in order to make clear the role of Sox9 in the genesis of chondrosarcoma. 目的构建Sox9siRNA表达载体,分析Sox9在软骨肉瘤中的作用机制。
- The expression cassette OAAT for multiple-epitope antigen of FMDV was successfully designed,and trivalent FMDV DNA vaccine pVAX1-OAAT was successfully constructed. 已成功设计FMDV复合多表位基因工程疫苗表达盒OAAT,并构建了三价口蹄疫核酸疫苗pVAX1-OAAT。
- And LipofectamineTM 2000 mediated gene transfection of p498 siRNA expression vector into COS-7 cells downregulated the mRNA and protein level of 4-1BB at 48h after transfection. 结果经测序分析;各m4-1BB siRNA表达载体构建成功; 其中p498 siRNA与p4-1BB双质粒转染COS-7细胞;48h后可观察到细胞表面4-1BB分子明显下调;其胞内mRNA水平也显著降低;与无关干扰组比较;差异有统计学意义(P<0.;05)。
- The invention further relates to an expression cassette comprising a useful polynucleotide placed under the control of a nucleic acid such as defined above. 本发明还涉及包含置于如上文所述核酸控制之下的目的多核苷酸的表达盒。
- Construction of Tim-1 siRNA expression vectors and its inhibitory effect on Tim-IHA expressionFour pairs of oligonucleotide targeting to Tim-1were designed and synthesized. 针对Tim-1的siRNA表达载体的构建及其对Tim-1HA的抑制效果
- However,until now,little is known about the function of pseudorabies virus(PRV) UL14.In this study,an UL14-negative PRV mutant(PRV-UL14D) was constructed by inserting an EGFP expression cassette into the UL14 coding sequence. 本研究以伪狂犬病毒鄂A株(PRV-Ea)为亲本株,通过同源重组,将编码EGFP的真核表达盒插入到伪狂犬病毒基因组的UL14基因中,PCR检测和荧光观察证实获得了能稳定表达EGFP的UL14插入失活突变株PRV-UL14D。
- HPV16 E7 specific siRNA expression vector can effectively inhibit the expression of E7 gene and partly reverse the malignant phenotype of CaSki cells,it may be one of the ideal strategies for gene therapy of cervical cancer. HPV16E7siRNA表达载体能有效地抑制宫颈癌CaSki细胞E7基因的表达,部分逆转其恶性表型。 因此它有望成为宫颈癌基因治疗的新策略之一。
- The sequences of RIP2 siRNA expressing plamid we had constructed was concordant with what we had designed. 2. 结果 1、构建的RIP2 siRNA表达质粒的序列与我们设计的一致。
- Keywords human bone sialoprotein;Pichia pastoris;expression cassette; 人骨唾液酸蛋白;毕赤酵母;表达盒;
- An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
- He always shows a doleful expression. 他总是露出忧郁的表情。
- I can think of no greater expression of defeatism. 这是我听到的最悲观的失败主义论调了。
- A constant, variable, function name, or expression. 一种常数、变量、函数名或表达式。
- She looked at me without expression. 她毫无表情地朝我看着。
- The scenery there was beautiful beyond expression. 那儿的风景美丽得无法形容。
- He looked at her with a solemn expression. 他表情严肃地望着她。
- There was an expression of discontent on her face. 她脸上有不满的表情。