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- Method:The NSCs were isolated from brain tissue of embryonic mice,then cultured in serum free medium,and identified by immunocytochemistry. 方法：胚胎小鼠脑组织分离神经干细胞，无血清细胞培养，免疫细胞荧光染色技术鉴定。
- Methods The expression of BDNF in cultured neurons were detected by immunohistochemistry after CM1 injected into serum free medium. 方法培养液加入一定剂量的CM1,利用免疫组织化学方法检测无血清培养神经元BDNF的表达。
- Among the SK-PLAR clones studied, SK-PLAR54 can differentiate neuron-like morphology upon 3 days culture in serum free medium. 然而，有报导基因表现的细胞株，其报导基因表现不受雄性素影响，呈现持续性表现。
- The positive granules for PDGF A and B chain mRNA expression were few in PASMC cultured in serum free medium(control group), but rich in PASMC exposed to PDGF BB polypeptide. 结果表明，无血清培养的肺动脉平滑肌细胞内PDGF?A和PDGF?B链mRNA阳性表达颗粒稀少，PDGF?BB多肽培养的肺动脉平滑肌细胞PDGF?A和PDGF?B链mRNA阳性表达颗粒增多，较密集的分布于整个细胞内。
- AIM To observe the effects of epidermal growth factor (EGF) and bovine pituitary extract (BPE) on human pituitary adenoma cells cultured in serum free medium. 目的 观察表皮生长因子 (EGF)和牛垂体提取物(BPE)在人垂体腺瘤细胞培养中的作用 .
- And results from TUNEL and trypan staining showed that both EGF and BPE of different concentrations decreased the number of apoptosis and death of human pituitary tumors cells cultured with serum free medium in vitro . 同时 ;EGF和BPE均可降低体外无血清培养的人垂体腺瘤细胞的死亡及凋亡数量 .
- The present study aimed to understand the effects of human UCB MSCs on the expansion of CD~+_(34) cells from UCB. Methods 1. Human UCB CD~+_(34) cells were incubated in the system containing UCB MSCs, HGFs and serum free medium. 方法(1)用含人脐血MSCs及不同造血生长因子(HGFs)组合的无血清扩增体系对人脐血CD+34细胞进行体外扩增。
- GF and BPE have an important role in human pituitary tumors cells cultured in serum free medium in vitro . EGF and BPE can promote the proliferation of human pituitary tumors cells cultured in vitro . EGF和BPE具有明显改善人垂体腺瘤细胞体外无血清培养条件的作用 ,EGF和BPE对离体培养的垂体腺瘤细胞增殖具有促进作用
- The Sika Deer splenocytes culture supernatant has action on the proliferation of NIH3T3 cells,and also can promote the wound healing of NIH3T3 cells. In the serum free medium this culture supernatant can also promote the proliferation of NIH3T3 cells. 梅花鹿脾细胞培养上清对正常小鼠 NIH3T3细胞具有促增殖作用 ,对创伤的 NIH3T3细胞具有促进愈合作用 ,并且在无血清培养液中此上清对正常 NIH3T3细胞也具有促增殖作用。
- Methods: Human BM-MSC from healthy donors and primary diagnosed patients with hematological diseases were cultured in DMEM/FBS or MesenCult serum free media. 方法:采用DMEM培养液加胎牛血清(DMEM/FBS)或无血清培养基体外培养人BM-MSC,测定细胞倍增时间;
- Use circs of the serum free medium 四、无血清培养基使用情况
- STUDY ON USE OF KSOM AS SERUM FREE MEDIUM IN OVINE EMBRYO CULTURE IN VITRO KSOM无血清培养液在绵羊胚胎体外培养应用的研究
- Primary study of tracheal membrane epithelium cells cultured by serum free medium 气管黏膜上皮细胞无血清培养的初步实验研究
- Serum free amino acid was measured before and after the treatment. 结果：经碳酸盐透析后，患者血浆大多数游离氨基酸和总氨基酸含量显著降低。
- serum free medium 无血清培养基
- Free media coverage, such as a news story or opinion piece. 传媒的免费报道，如一条新闻或一篇评价文章。
- Even in euthyroid subjects with normal serum TSH levels, serum free T4 concentration is independently associated with AF. 即使在有正常促甲状腺激素刺激激素（TSH）水平的正常甲状腺功能受试者，血清游离T4浓度也是与房颤相关的独立因素。
- The dialysis with balancing amino acids might be useful to prevent part loss of serum free amino acid. 结论：平衡型氨基酸透析液能减少血中部分氨基酸的丢失。
- Production of ECCM was obtained from serum free DMEM with or without CyA(0,50,100,300,500,1000 ng/ml). 用无血浆DMEM细胞培养液制备含或不含CyA(0、50、100、300、5001、000 ng/ml)的ECs调节的细胞培养液;
- Methods The technique of serum free cultivation and the methods of immunofluorescence cytochemistry were used. 方法 利用无血清培养技术和细胞免疫化学方法。
