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- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- Methods The expression of MDR1 gene in 77 cases of AL patients and 10 cases of control group was detected with semi quantitative reverse transcription polymerase chain reaction (RT PCR). 方法 采用半定量逆转录聚合酶链反应（ R T? P C R），对77 例 A L病人及 10 例正常人 M D R1 基因表达进行检测。
- It can give the qualitative, semi quantitative, or quantitative data about the compositions of fluid inclusions quickly. 它可以快速方便地对单个包裹体进行定性、半定量乃至定量分析,且样品制备简单。
- Objective To construct and evaluate the competitive quantitative RT PCR method for detecting the fusion gene of AML1 ETO in the patients of t(8;21)AML(acute myeloid leukemia). 目的 建立针对 AML- ETO融合基因转录本的竞争性定量反向 -聚合酶链反应 (RT- PCR)体系 ,并探讨其在 t(8;2 1)急性髓细胞性白血病 (acute myeloid leukemia,AML)微小残留病变跟踪中的应用价值。
- The levels of Prx and TrxR mRNA were determined 24 h and 48 h post,transfection by relative quantitative RT,PCR,and the growth of Trichomonas vaginalis was estimated under microscope 36 h post,transfection. 收集转染前、转染后24 h和48 h的毛滴虫细胞,用半定量反转录-PCR(RT-PCR)检测Prx和TrxR的mRNA水平;
- With semi quantitative noncompetitive RT-PCR assay, the hTERT mRNA expression in IMR90 cells was negative and the normalized hTERT mRNA expression in T24 cells was 0.06[hTERT(IOD)/GAPDH(IOD)]. 半定量非竞争RT-PCR检测IMR90和T24细胞株hTERT mRNA的表达，IMR90细胞株hTERT mRNA无表达，T24细胞株hTERT mRNA表达为0.;06[hTERT(IOD)/GAPDH(IOD)]。
- effect of As ODN on ppET 1 mRNA expression of GMC was measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR); 用半定量反转录聚合酶链反应 (reversetranscription polymerasechainreaction ,RT PCR)检测寡核苷酸链对GMC的 ppET 1mRNA表达的影响 ;
- Polymerase chain reaction (PC R) 聚合酶链反应
- Fluorescent Quantitative RT - PCR 荧光定量RT-PCR
- real - time quantitative RT - PCR 实时定量RT-PCR
- real-time fluorescence quantitative RT - PCR 实时荧光定量RT-PCR
- Real - time fluorescence quantitative RT - PCR 实时荧光定量PCR
- Polycyclic aromatic hydrocarbon (PAH) fractions in airborn particulates were quantitatively and semi quantitatively analyzed by GC MS.16 kinds of parent PAH were detected in airborn particulates. 用GC-MS定量和半定量分析了PAH组分;研究区大气飘尘中检测出16种母核PAH.
- Real-time quantitative PC R 实时定量PCR
- Maintenance of PC is in my element. 我对电脑的维修保养很在行。
- Research on the Appliance Extent of "Source Control Theory" by Semi quantitative Statistics Characteristics of Oil and Gas Migration Distance "源控论"适用范围量化分析
- Having no fixed quantitative value. 变量的没有固定数量值的
- nested PC R 巢式PCR
- and AcrE was synthesized by 12 ploymerase chain reactions. Construction of internal standard of quantitative RT - PCR with synthesis method was quickly and easily accomplished. 合成法构建定量RT-PCR内标准DNA快捷、成功率高。
- It steamrollers for us to own and use PC's. 对我们来说，拥有并使用个人微机已成不可阻挡之势。
