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- One RING domain and two BIRs domain contained in IAP2 while one RING and one BIR domain contained in IAP3.iap2 and iap3 were ligated into expression vector pET-28a, then were transformed into BL21(DE3) competent cell respectively. 用表达载体pET28a-iap2转化宿主菌BL21(DE3)感受态细胞,挑取单菌落并接种于加卡那霉素的LB中,扩大培养后加入IPTG诱导表达,表达结果显示,含iap2基因载体的表达产物在电泳中出现一条约40kDa的表达带,与理论推测的蛋白分子量一致。
- She had the sapphire set in a gold ring. 她把那枚蓝宝石镶在金戒指上了。
- The diamond ring dazzled with brilliancy. 这只钻石戒指闪耀夺目。
- Military history is really outside my domain. 我对军队史实在一窍不通。
- She wears a diamond ring on her left hand. 她左手上戴着一枚钻石戒指。
- Our family reunions are always three ring circus. 我家合家团聚的场面总是热闹非凡。
- I am sorry-she's out. Can you ring again later? 对不起-她出去了。你过些时候再打电话来好吗?
- The boys and girls made a ring round us. 男孩和女孩围着我们站了一圈。
- Please ring for the attendant if you need anything. 如果需要什么,请按铃找服务员。
- The music was so loud it made my ears ring. 音乐的声音太大了,把我耳朵震得直响。
- The policeman felt that my story didn't ring true. 警察认为我讲的话听起来不可信。
- She wears a ring round her wrist. 她的手腕上戴着一只镯子。
- Ring the correct answer with your pencil. 用铅笔圈出正确的答案。
- The kitchen is my wife's domain, she doesn't like me going into it. 厨房是我妻子的领地,她不愿让我进去。
- A pull on the rope will make the bell ring. 一拉绳子钟就响。
- The conspirators were banished from the king's domain. 阴谋家们被赶出了国王的领土。
- He have scarcely get home when the telephone ring. 他一回到家电话铃就响了起来。
- I'll ring you up some time during the week. 本周的某个时候我给你打电话。
- The jeweller will engrave the inside of the ring with her name. 珠宝匠将在戒指的内表面上刻上她的名字。
- He can't get his ring off his finger. 他无法指导戒指从手指上拿下来。