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- The Restriction Enzyme Database from NEB. 限制苺资料库取自新英格兰生物实验室公司。
- It enables a specific gene to be located on a particular restriction enzyme fragment. 它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。
- Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation. 根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。
- Certain DNA sequences oriented within this gap are substrates for Alu I, a blunt end restriction enzyme. 如果用一段酶切断作为桥梁的DNA，那么整个电路的电流也被中断了。
- Amplified of the green fluent protein (GFP) gene from plasmid PCR3.1, and add suitable restriction enzyme cutting site. 从含有gfp基因的质粒pCR3.;1上扩增出全长基因，并且加上合适的酶切位点。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- The expressive vector pcDNA3.1(-)-ASL has been constructed and had been confirmed by restriction enzyme cut and sequencing. 构建的真核表达载体pcDNA3 .;1(-) ASL经过酶切鉴定和测序证实正确。
- Genomic DNA was extracted.Polymerase chain reaction, direct sequencing and restriction enzyme reaction were performed to identify the mutations. 利用聚合酶链反应、DNA直接测序进行突变检测,进一步采用限制性内切酶酶切验证突变。
- Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。
- The vector was identified via PCR and restriction enzyme analysis and was introduced into Agrobacterium tumerifacience LBA4404. 经限制性内切酶分析和PCR鉴定后利用冻融法将重组质粒pBI121-A导入根癌农杆菌LBA4404。以锦橙 (Citrus.
- The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP). 用聚合酶链反应-限制性片段长度多态性方法,分析限制性酶切位点-48A/的多态分布。
- Methods: The serum samples were collected from 56 patients with morbilliform maculopapular eruption.The target DNA of CBV was detected by RT-PCR and restriction enzyme analysis. 方法:取56例春季流行性麻疹样发疹患者外周血标本,扩增目的DNA并进行特定限制性内切酶分析。
- Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 结果 1、对重组载体的酶切鉴定和测序结果证实，在pS2-DTA中EWS-FLI-1特异性结合序列(ACCGGAAGT)位于白喉毒素A链基因上游。
- The ORF of two circadian gene BMAL1 and CRY1 were cloned from normal human whole brain marathon cDNA and verified with restriction enzyme digestion and DNA sequencing. 从正常人全脑marathon cDNA中克隆到人生物节律基因BMAL1和CRY1的开放读码框架(ORF)，经酶切和测序鉴定与GeneBank数据库中收录的序列基本一致。
- Restriction endonuclease (restriction enzyme) A type of enzyme, found mainly in bacteria, that can cleave and fragment DNA internally(see endonuclease). 限制性内切酶（限制酶）：主要在细菌中存在的一种酶类，它可以从DNA的内部将其切开和分裂。
- Methods Polymerase chain reaction with restrict enzyme digestion was used to detect the target gene polymorphism in 78 normotension controls and 72 EH patients. 目前认为，高血压是多种遗传因素和环境因素共同作用而引起的复杂性状疾病，在人类遗传易感性的基础上，在环境危险因素的作用下发病。
- New phages can develop by acquiring restriction enzymes from plasmids. 通过从质粒获得的限制性酶,新的噬菌体能够生长。
- Cut outside restricted enzymatic what be? 限制性外切酶是什么呀？
- Restriction enzymes and reverse transcriptase are keys to gene engineering. 限制性酶和逆转录酶成为基因工程的重要手段。
- Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site. 以两条部分重叠长链引物P_2和P_3延伸产物为模板，P_1和P_4为正、反引物进行PCR扩增，获得了改造的抗菌肽基因SPCEMA(187bp)。
