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- restriction endonuclease assay 限制性酶切分析
- A ,sad sequence ;B ,restriction endonuclease cutting sites of sad. 段,与序列分析结果相一致(图略)。
- Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid. 方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法。
- Mimics of both types of BCR ABL cDNA were achieved and the validity was verified with restriction endonuclease. 经酶切分析证明 ,两型BCR ABLmRNA均可通过此方法得到相应的cDNA参照物。
- Results The result of restriction endonuclease digestion was accordance with the anticipated objective strap size . 结果 酶切结果与预期目的条带大小相符；
- PCR amplification and restriction endonuclease digestion was used to identify deleted DNA fragments. 应用PCR技术与限制性内切酶酶切相结合的方法鉴定缺失子。
- Method The two clones of OSCP gene were modified by restriction endonuclease and recombined by T_4DNA ligase. 方法通过限制性核酸内切酶将发生有义突变的两个OSCP基因的DNA克隆进行改造,并进行基因重组。
- The 344C/T polymorphism of CYP11B2 gene was monitored by PCR and HaeIII restriction endonuclease digestion methods. 应用多聚酶链式反应(PCR)、限制性内切酶方法检测CYP11B2基因的多态性分布。
- After the PCR amplicons of SEE strain sere eleaved by restriction endonuclease EcoRV.electrophofretic analysis showed two 251 and 415bp DNA fragments. SEE菌株的扩增产物经EcoRV酶切能产生251和415bp两个片段。
- A portion of Ligation products were identified by BamHI and Hin-dIII restriction endonuclease and was named pUC - B2 - AR. 2·PMCX－p。 －AR反义表达载体的酶切鉴定，其结果与理论设计完全相符。
- The recombinant plasmid pAd-K14-E6/E7-polA was successfully constructed and confirmed by restriction endonuclease digestion and sequencing. 通过同源重组的方法构建了腺病毒pAd-K14-E6/E7-polA载体,经酶切和测序鉴定该质粒构建成功。
- Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful. 结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。
- The recombinant plasmid PsecTaq2A_AMG was analyzed by restriction endonuclease mapping and DNA sequencing. The results of sequencing were consistent with those from GenBank. 重组克隆PsecTaq2A_AMG酶谱分析与预期结果一致 ,序列测定结果与GenBank中的人釉原蛋白序列完全一致。
- Secondly,the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends,which were then ligated to a designed DNA adapter by ligase. 然后用限制性内切酶将其消化成短片段,在连接酶的作用下与设计的DNA适配器相连;
- The PCR products were cloned into T clone vector,and the positive clones were picked out,then the T clone vector was identified by restriction endonuclease digestion. 将扩增的连接产物克隆入T载体，挑选阳性克隆并进行酶切鉴定，酶切鉴定正确的克隆进行拼接产物的核苷酸序列测序。
- Restriction endonuclease (restriction enzyme) A type of enzyme, found mainly in bacteria, that can cleave and fragment DNA internally(see endonuclease). 限制性内切酶（限制酶）：主要在细菌中存在的一种酶类，它可以从DNA的内部将其切开和分裂。
- After identification with restriction endonuclease digestion, sequencing, and indirect immunofluorescence (IF), the suicidal DNA vaccine (pAHSP65) was inoculated into mice. 经酶切、测序鉴定,间接免疫荧光(IF)证实其能表达HSP65后,将此“自杀性”DNA疫苗(pAHSP65)免疫小鼠。
- Restriction endonuclease digestive identification was right for recombinant expression vector pCD11b BFP. Blue fluorescence from fusion protein could be seen in U937 cells transfected with plasmid pCD11b BFP. 经酶切鉴定 ,p CD11b- BFP构建完全正确 ,转染 U937细胞株后 ,可见 CD11b- BFP融合蛋白发出的蓝色荧光。
- The OmpL1 gene of Leptospira(L.) serovar lai was amplified and sequenced, and its nucleotide sequence, protein secondary structure and restriction endonuclease map were further analysed. 用PCR方法扩增不同毒力赖型钩体OmpL1基因片段，进行序列测定，用相关软件比较分析核苷酸序列、蛋白质二级结构以及限制性内切酶谱。
- Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism. 方法用盐提取法提取人群中白细胞的DNA,以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。
