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- Chunna Yu, Shuqing Chen, Su Zeng. Reporter Gene Assay to predict and Rank the Inducibility of CYP3A4 by TCMs. Asian Journal of Pharmacodynamics and Pharmacokinetics, 2006, 6 (3): 250. 俞春娜,陈枢青,曾苏,采用报告基因分析法预测中药对CYP3A4的诱导作用,第八次全国药物与化学异物代谢学术会议,大连,2006年10月。
- PCR analysis and histochemical assay of GUS activity are conducted to testify the transformation events and the successful expression of reporter gene in independent hygromycin resistant calli. 为建立银杏遗传转化的高效体系,对一些重要的影响银杏转化的因素,包括外植体类型,选择标记和筛选压,农杆菌工程菌株,共培养时间和恢复时间第一次进行了试验并优化了条件。
- Based on the above, we constructed two strains of controllable recombinant baculovirus which bring TRE and reporter gene expression system, with different promoters to express TetR. 在此基础上构建了两株用不同启动子表达tet阻遏蛋白,并同时带有tet响应元件及报道基因表达系统的完整的tet调控系统的重组杆状病毒,构成可调控表达的重组杆状病毒。
- Thus we constructed reporter vectors in which the reporter gene is luciferase. 经酶切鉴定以及测序分析确定载体构建正确。
- Androgen Receptor Reporter Gene Assay 雄激素受体报告基因实验
- Methods Based on the suicide vector pGP704,a new screening in vivo-indu ced genes vector pGPcat was constructed with chloramphenicol a cetyltransferase(cat)gene as a report gene. 方法以自杀载体pGP704为骨架,用氯霉素乙酰转移酶基因作为报告基因,构建了用于筛选体内诱导基因的载体pGP-cat。
- Method:We detected telomerase activity of HEP 2 cell line,26 cases of supraglottic carcinoma tissues and 15 cases of peri carcinoma tissues with PCR based assay designated TRAP(for telomeric repeat amplification protocol). 方法:采用端粒重复序列扩增法检测了 H E P?2 喉癌细胞系,26 例声门上型喉癌组织和15 例癌旁组织。 结果: H E P?2 喉癌细胞系呈端粒酶阳性;
- Prof. Yangtao:The genes based on the susceptibility, can you comment on this? 杨涛教授:请您具体的说说易感基因是怎么回事?
- SuperLight Luciferase Reporter Gene Assays for gene regulation and modulator screen. 荧光素酶报告基因检测试剂盒,用于基因调控筛查的。
- Seed chip GUS assay and herbicide leaf painting and spraying assays were applied to test the gus reporter gene and the herbicide resistant bar selectable marker gene, respectively. 分别采用种子切片GUS染色方法和除草剂涂抹以及喷洒方法检测gus报告基因和抗除草剂bar基因在后代的表达。
- This promoter is shown to direct the specific expression of the reporter gene,B-glucuronidase(GUS), in trichomes of Arabidopsis. 将此启动子与gus报告基因相连构建植物表达载体并转基因拟南芥,GUS组织化学定位分析表明此启动子在拟南芥叶片和茎的表皮毛细胞中特异表达。
- The transient transfection of Stat 3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. Stat3cDNA和报道基因在COS7细胞中的共转染实验表明 ,外源的Stat3蛋白具有DNA结合活性 ,并可增强报道基因的表达。
- It belonged to the TIR-NBS-LRR resistance gene based on putative amino acid sequence and contained all of conservative domains of TIR-NBS-LRR resistance genes. 根据推测的氨基酸序列,该基因属于TIR-NBS-LRR类抗病基因,具有TIR-NBS-LRR类抗病基因所有的保守结构域。
- Internationa Diabetes: The genes based on the susceptibility, can you comment on this? 国际糖尿病:请您具体的说说易感基因是怎么回事?
- Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. 将启动子片段分别以不同方向与gus报告基因和nos终止子融合 ,构建了瞬时表达载体。
- Identification of Anthracnose Resistant Genes Based on SCAR Markers in Common Bean (Phaseolus vulgarisL. 关键词:普通菜豆;菜豆炭疽病;抗病基因;分子标记;SCAR
- Transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and also the reporter gene expression. Stat3 cDNA和报告基因在COS7细胞中的共转染实验表明,外源的Stat3蛋白具有DNA结合活性,并可增强报告基因的表达。
- Objective To search an effective method on screening significant genes based on microarray data. 目的基于微阵列表达数据,探索筛选差异表达基因的有效方法。
- Reporter gene imaging is an important imaging technique in molecular imaging,which can image various biological and genetical processes. 报告基因显像技术是分子影像学中重要的显像技术,其可对多种不同的生物学和分子遗传学过程进行显像,有望更快地应用于临床。
- The minor T allele significantly attenuates reporter gene expression (P<0.01) and is impaired in its capacity to form DNA-protein complexes (P<0.05). 该等位基因处如为T可使基因表达量显著下降,并损伤其形成DNA-蛋白复合体的能力。
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries