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- The applications in molecular biology are mainly report gene &molecular hybridization.A real-time bioluminescent DNA sequencing system was also developed. 分子生物学领域内的应用主要为报告基因和分子杂交 ,近年来又有人推出了生物发光实时DNA测序技术。
- In this thesis, we demonstrate that sarA regulates the transcriptions of lipA, zinc and atlE in S. epidermidis by reverse-transcription PCR and report gene lacZ analysis. 本文利用RT-PCR方法和LacZ报告基因系统，证实了表皮葡萄球菌中sarA基因对自溶酶基因（atlE），脂肪酶基因（lipA）和膜相关锌金属蛋白酶基因（zinC）的表达分别具有直接的抑制作用，促进作用和促进作用。
- Thus we constructed reporter vectors in which the reporter gene is luciferase. 经酶切鉴定以及测序分析确定载体构建正确。
- To genenatethe pAAV-LacZ viral particles, using LacZ as a report gene, AAV-293 cells wasco-transfected with the three plasmids(pAAV-LacZ, pAAV-RC, and pHelper) bythe way of liposome-mediated transfection. 脂质体共转染法在AAV-293 细胞中包装生成以LacZ 为报告基因的pAAV-LacZ 病毒颗粒;
- Objective To construct spinocerebellar ataxia 3 (SCA3) eukarotic expression vector with enhanced green fluorescent protein (EGFP) as report gene and to observe its expression in PC12 cells. 目的构建以增强型绿色荧光蛋白(EGFP)作为报告基因、带有脊髓小脑性共济失调3型(SCA3)基因的真核细胞表达载体,并转染PC12细胞,观察外源基因的表达情况。
- There are three kinds of biosensors relevant to bioluminescence &chemiluminescence: immobilized enzyme sensor, immunoassay sensor and report gene sensor with specialized regulator. 基于生物及化学发光的生物传感器主要有三类 :酶固化传感器、免疫分子传感器和特异调控因子诱导的报告基因传感器。
- Fig.1 The Construction of plant transformation vector of anti-sense gene of RbThe transcript director of Rb is opposite to 35S promoter.Thisvector has GUS report gene and hygromycin resistance gene. 标题： 图1 Rb反义基因表达载体结构示意图此表达载体Rb的转录方向与35S启动子的转录方向相反,并且含有GUS报道基因和抗潮霉素基因。
- The main task for post-doctorate is molecular imaging research for hepatic diseases, especially in the fields of report gene, plasmid construction, and monocrystalline iron oxide nanocompound (MION). 肝脏疾病的分子影像学研究，注重于报告基因、质粒构建和纳米单晶磁性粒子的研究。
- Methods Based on the suicide vector pGP704,a new screening in vivo-indu ced genes vector pGPcat was constructed with chloramphenicol a cetyltransferase(cat)gene as a report gene. 方法以自杀载体pGP704为骨架,用氯霉素乙酰转移酶基因作为报告基因,构建了用于筛选体内诱导基因的载体pGP-cat。
- SuperLight Luciferase Reporter Gene Assays for gene regulation and modulator screen. 荧光素酶报告基因检测试剂盒,用于基因调控筛查的。
- This promoter is shown to direct the specific expression of the reporter gene,B-glucuronidase(GUS), in trichomes of Arabidopsis. 将此启动子与gus报告基因相连构建植物表达载体并转基因拟南芥，GUS组织化学定位分析表明此启动子在拟南芥叶片和茎的表皮毛细胞中特异表达。
- I was frantic to finish the report on schedule. 我十万火急地要按预定时间完成报告。
- The transient transfection of Stat 3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. Stat3cDNA和报道基因在COS7细胞中的共转染实验表明 ,外源的Stat3蛋白具有DNA结合活性 ,并可增强报道基因的表达。
- Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation. 将启动子片段分别以不同方向与gus报告基因和nos终止子融合 ,构建了瞬时表达载体。
- He claimed that the newspaper report was a libel. 他声称报纸上的报导是一种诽谤。
- Transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and also the reporter gene expression. Stat3 cDNA和报告基因在COS7细胞中的共转染实验表明，外源的Stat3蛋白具有DNA结合活性，并可增强报告基因的表达。
- Reporter gene imaging is an important imaging technique in molecular imaging,which can image various biological and genetical processes. 报告基因显像技术是分子影像学中重要的显像技术,其可对多种不同的生物学和分子遗传学过程进行显像,有望更快地应用于临床。
- How can I worm out of giving that report? 我怎么逃脱不做那个报告呢？
- He is reading a report of the state of the roads. 他正在看一篇关于道路状况的报告。
- The minor T allele significantly attenuates reporter gene expression (P<0.01) and is impaired in its capacity to form DNA-protein complexes (P<0.05). 该等位基因处如为T可使基因表达量显著下降，并损伤其形成DNA-蛋白复合体的能力。
- 今日热词
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries
