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- The amplified gene was cloned into the pENTR11 vector. With the pENTR11-SLC plasmid and the backbone plasmid pAd/CMV/V5-DEST, the homologous recombination reaction took place in vitro. The reaction mixture was transferred into TOP10 E. 方法采用PCR技术从含有SLC基因的质粒上扩增出SLC基因,将PCR产物酶切后连接至pENTR11载体上,再通过pENTR11与腺病毒骨架载体pAd/CMV/V5-DEST之间的同源重组作用将SLC基因片段重组至pAd/CMV/V5-DEST上,最后经293细胞的包装扩增后得到携带SLC基因的重组腺病毒。
- By the BP recombination reaction,the PCR product containing attB was transfered to an attP-containing donor vector to create an entry clone. Finally,DREB1A gene was shutted into pH2GW7 vector by LR recombination reaction. 通过BP反应将包含有attB接头的PCR产物克隆到含有attP的donor载体上以产生Entry克隆,通过LR反应将已经重组入Entry载体的DREB1A基因再克隆到pH2GW7双元载体。
- Keywords reaction kinetics;recombination reaction;oxirane; 反应动力学;复合反应;环氧乙烷;
- Could I ask for your reaction to this policy? 我能问一下您对这个政策的反应吗?
- Construction of cDNA library of B. Cinerea by in vitro site-specific recombination reaction 利用体外特异位点重组反应构建黄瓜灰霉菌cDNA文库
- A reactor is a container for chemical reaction. 反应器是进行化学反应的容器。
- I'm displeased at your reaction. 我对你的反应很不满意。
- The forces of reaction made reform difficult. 反动势力给改革造成了困难。
- His reaction on this matter is unexpected. 没料到,他对这件事的反应是这样的。
- A sharp reaction; a repercussion. 强烈反应后果,影响
- Their reaction was one of qualified praise. 他们的反应是一种有保留的称赞。
- His initial reaction was one of shock. 他最初的反应是震惊。
- An enzyme that catalyzes genetic recombination. 催化重级酶能催化基因重组的一种酶
- The reaction of the audience was instantaneous. 观众即刻有了反应。
- What was his reaction to the plan? 他对这个计划有什么反应?
- The medicine produced a violent reaction. 这药物引起了剧烈的反应。
- My reaction will be to partake in the speech competition. 我的看法倒是要参加这次演说比赛。
- What was his reaction to the news? 他对这消息的反应如何?
- His reaction of swiftness surprised us. 他快速的反应能力让我们吃了一惊。
- I had a bad reaction after my typhoid injection. 我注射了伤寒针剂后产生了不良反应。