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- We used the purified recombinant protein expressed from prokaryotic cells to immunize the New Zealand whit rabbit and achieve the high titer and superiority antisera. 以前研究发现PRRSV、IBV、MHV和TGEV等冠状病毒的N蛋白都定位在细胞质与核仁中,这可能是冠状病毒的共有特征。
- Mice were used to determine the hypocalcemic effect of the recombinant protein.Results showed that the recombinant protein expressed in E.coli had the proper bioactivity. 利用小鼠降血钙实验检测变性复性后产物的活性,结果表明该重组蛋白有一定的生物活性。
- Objective To observe the protective effict of hepatitis E virus(HEV)ORF 2 recombinant protein expressed in prokaryote cell cynomolgus macaques(cynos)against challenging with wild-type HEV. 目的观察基因重组戊型肝炎病毒(HEV)结构蛋白对恒河猴戊型肝炎野病毒攻击的保护作用。
- This method can be used in research on protein unfolding/refolding and seeking for refolding protocol of recombinant proteins expressed in bacteria. 可用于蛋白质变性/复性的研究及原核基因工程产品复性工艺的探索。
- The Expression Analysis of Recombinant Protein in Escherichia coli. 大肠杆菌中表达外源重组蛋白的研究。
- Cloning, expression and purification of recombinant protein. 运用常规分子生物学与生化技术克隆表达与纯化蛋白质。
- recombinant protein expression 体外蛋白表达
- The expression analysis of recombinant protein in \%Escherichia coli BL21 (DE3). 大肠杆菌BL21(DE3)中表达重组蛋白的研究
- It turns that most of the recombinant protein were inclusion body. 对重组蛋白的细胞内定位分析发现蛋白几乎全部以包涵体形式存在。
- The secretion of recombinant protein to periplasm or extracellular medium has more advantages than the expression intracellular as inclusion bodies. 重组蛋白分泌到周质空间或胞外培养基中较之在胞内以包含体形式表达有许多优势。
- Objective To clone human ornithine decarboxylase antizyme (OAZ) mutation gene and express its recombinant protein. 目的构建人鸟氨酸脱羧酶抗酶(OAZ)突变基因,重组至原核表达载体中并表达出重组蛋白。
- The protein expression of Bax was increased after DATS treatment. 免疫组化显示;经DATS处理后;Bax的表达增强(P<0.;05);Bcl-2表达未见明显改变。
- Under the optimized expression conditions, we got a lot of recombinant protein, which then was purified by using Ni-NTA chelating affinity chromatography. 在最佳表达条件下大量表达蛋白,获得大量的重组蛋白后,使用Ni-NTA螯合亲和层析的方法对其进行纯化。
- Progress on RecomBination Protein Expression and Sub-unit Vaccine of Foot-and-mouth Disease Virus 口蹄疫病毒重组蛋白表达及其亚单位疫苗研究进展
- The engineering strain was obtained by screening, it"s induced expression condition was optimized.The recombinant protein hIL-10 was overexpressed in E.coli stably. 筛选工程菌,优化诱导表达条件,在大肠杆菌中获得稳定高效表达。
- The E. coli strain BL21(DE3) transformed by pETOMP25 could express a recombinant protein with reactivity,which amounts to 18.6% in the total protein of the induced bacteria. 转化重组质粒pETOMP25的大肠杆菌BL21(DE3)在IPTG的诱导下;可表达出具有反应原性的目的蛋白;表达量占菌体蛋白的18.;6%25。
- The positive rate of COX-2 protein expression was 61.67% in NPC tissues. COX-2在NPC中总的阳性表达率为61.;67%25。
- The bacterium Escherichia coli is a widely used host for protein expression. 大肠杆菌被广泛应用于蛋白表达的宿主。
- Green florescent protein allows the identification of protein expression. 萤光绿蛋白可以用作检测蛋白表达。
- Results: VEGF protein expression was detected in 32 of 57 human gliomas. 结果:在57例胶质瘤中32例观查到了VEGF蛋白表达。
- 今日热词
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- 深远海浮式风电平台 - deep-sea floating wind power platform
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- 京雄高速公路 - Beijing-Xiongan expressway
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- 农业及相关产业增加值 - the added value of agriculture and related industries