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- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- The whole FC cDNA was cloned into pET-28a (+) containing T7 promoter and recombinant expression plasmid pET-FC was constructed. The recombinant plasmid was transformed into E. coli BL21(DE3). 将分段克隆得到的两段FC片段经相应酶切后 ,与含T7启动子的质粒 pET 2 8a(+)在一个体系中作连接反应 ,构建表达质粒pET2 8a FC ,转化大肠杆菌BL2 1(DE3) ,筛选表达菌株。
- The result of immunologic test showed that there was crossed protection between the two bacteria.Vanhnaseng(2004) cloned the gene of cIL-18 and obtained recombinant expression plasmid pcDNA3.1/cIL-18. 曹素芳（2004）克隆了禽巴氏杆菌外膜蛋白H基因（ompH），构建了该基因与cIL-18基因真核共表达质粒pcDNA3.;1/ompH-cIL-18，免疫试验结果表明，pcDNA3
- A recombinant expressing plasmid pCMV4/TPO was also constructed by cloning the rhTPO cDNA in an eukaryotic expressing vector pCMV4. When the recombinant plasmid was used to transfect the COS7 cells, temporary expression of rhTPO was detected. 将TPOcDNA亚克隆至哺乳动物细胞瞬时表达载体pCMV4，形成了重组表达质粒pCMV4/TPO。 以该重组质粒转染COS7细胞，可测到TPO在COS7细胞中的瞬时表达
- Methods Murine IL 12 cDNA was inserted into optimized HBcAg DNA vaccine pST HBc. The resultant recombinant expression plasmid pST HBc/IL12 was transfected into COS7 cells and its expressed product was detected by ELISA. 方法 将小鼠IL 12基因插入结构优化的DNA疫苗pST HBc,构成pST HBc/IL12 ,将pST HBc/IL12转染COS7细胞 ,ELISA检测培养上清中的HBcAg和小鼠IL 12。
- Plant recombinant expression plasmid 植物表达载体
- The results showed that eaeA gene was correctly inserted into the pET-28a(+) vector, and the recombinant expression plasmids (named pET-eaeA) were constructed successfully. 将经质粒PCR鉴定和双酶切鉴定为阳性的转化子测序作进一步鉴定，成功构建了重组表达质粒pET-eaeA。
- For the first time to recombine ALR procaryotic expression vector, and get the pET28a-hALR recombined expressing plasmid which was tested by sequence analysis. 首次构建了重组ALR原核表达载体，获得了pET28a-hALR的重组表达质粒，并测序证实。
- The sequences coding two target proteins were cloned into the above recombinant expression plasmids separately and transfected into Cos 7 cells mediated via liposome. The expression products were detected by Western blot and co immunoprecipitation. 将两种候选蛋白的编码序列分别克隆于上述融合载体,转染Cos?7细胞后的表达产物,可利用抗HA或myc的mAb进行Western?blot和免疫共沉淀。
- STⅡ plant recombination expression plasmid 植物重组表达质粒
- Construction of the Recombinant Expression Plasmid for the Expression of the Whole Anti-D Immunoglobulin Molecules 完整人抗-D抗体分子表达载体的构建
- Identification of recombinant expression plasmid by restriction endonuclease digestion 重组表达质粒酶切鉴定
- A STUDY OF GENE IMMUNIZATION WITH RECOMBINANT EXPRESSION PLASMID OF EPSTEIN-BARR VIRUS MEMBR ANEANTIGEN 含有Epstein-Barr病毒膜抗原的重组表达质粒及其基因免疫
- Keywords human herpesvirus 8;K12 gene;green fluoresent protein;recombinant expression plasmid; 关键词人疱疹病毒8型;K12基因;绿色荧光蛋白;重组表达质粒;
- Keywords Oenococcus oeni;malolactic enzyme gene;malate permease gene;recombinant expression plasmid;expression;Saccharomyces cerevisiea; 酒酒球菌;苹果酸-乳酸酶基因;苹果酸通透酶基因;重组表达质粒;表达;酿酒酵母;
- recombinant expression plasmid 重组表达质粒
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- Expression and purification of recombinant RTAsThe expression plasmid Pkk223.3-RTA was introduced into E. coli JM 109 by CaCl2-mediated method. 将构建好的重组质粒pKK223.;3-RTA和pKK223
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
