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- Establishment of the Recombinant Yeast Assay System for Environmental Estrogens 环境雌激素重组酵母测评系统的建立
- recombinant yeast assay system 重组酵母测评系统
- Keywords Water pollution;Cell proliferation assay;Recombined yeast assay;Reclaimed water;Estrogenic effect; 水污染;细胞增殖试验;基因重组酵母试验;再生水;雌激素效应;
- Method: The seeds of Camellia japonica extract was separated into four fractions according polarity, its antiosteoporosis effective parts was investigated by a recombinant yeast screening assay. 方法：用系统溶剂分离法将南山茶子醇提物分成极性不同的组分，利用重组人雌激素受体基因酵母生物检测法筛选山茶抗骨质疏松的有效部位。
- The recombinant yeast strain could grow well on cellobiose as a sole carbon source and could be us. 酒精发酵的糟液中含有一定量的糖未能被酿酒酵母有效利用,其中最多的是蜜二糖和纤维二糖。
- recombinant yeast estrogen system 重组酵母测评系统
- CHEN Xiuyun,CHEN Chunpo,WU Vujuan,et al.Study on the estrogenic activities of DDT pesticides based on the gene recombinant yeast cell[J].J Toxicol,2006,20(6):377-379. [2]陈秀云;陈春波;吴玉娟;等.;重组基因酵母细胞对DDT类农药的雌激素活性研究[J]
- The most chemically satisfying approach may seem to require the use of the assay system. 化学上最理想的探讨途径，似乎需要应用一些鉴定系统。
- A clear zone surrounding the colony of the recombinant yeast was formed on methanol medium containing tannin indicating the presence of tannase secreted by the recombinant. 单宁酶在真核系统的表达表现出很大的潜力，相比天然单宁酶的产量低、生产成本高的缺点，利用高效的表达系统生产重组单宁酶有着广阔的前景。
- To address this question, we developed an in vitro assay system to evaluate the molecular mechanism of amelogenesis. 为了解决这一问题，我们使用一种体外测定系统来评估釉质发生的分子机制。
- Therefore it is recommended to combine the yeast assay with the metabolic activation method based on H4IIE cell line to assay the direct and indirect estrogenic chemicals in environmental samples. 因此，推荐将鼠肝癌细胞代谢活化与重组基因酵母检测的方法相结合，用于环境样品中直接和间接雌激素效应物质的检测。
- Procedures used in the assay system configuration code, the CPU speed detection code for compilation mixed language programming. 程序中使用了部分检测系统配置的代码，检测CPU速度的代码为汇编语言混合编程。
- We have applied both the conventional ERE-luciferase reporter assay system and the GAL4-transactivation system to characterize this receptor. 我们既适用于传统的东铁支线-荧光素酶检测系统和gal4 -激活系统，以特有的这种受体。
- Using the assay system of Ca2+-dependent cyclic nucleotide phosphodieste-rase (PDE), we observed the antagonistic effects of ligusterazine on calmodu-lin (CaM) . 实验利用Ca~(2+)依赖性环核苷酸磷酸二酯酶(PDE)测定方法,观察川芎嗪拮抗钙调素(CaM)的作用。
- Methods Using the assay system of peroxide value (POV), diphenyl picryl-hydrazyl (DPPH), the antioxidative activities of various extracts were studied and comparedwith VE and VC. 方法以过氧化值(POV)法和DPPH法对提取物进行抗氧化活性的测定,以维生素E(VE)和维生素C(VC)作抗氧化性能对照。
- Methods The relative quality of dust deposited in pulmonary intersititum of silicotic rats was evaluated by using polarizing microscopy and image assay system. 方法采用偏振光显微技术和图像分析系统，评价染尘大鼠肺组织滞留粉尘的相对量。
- Recombinant yeast -derived hepatitis B vaccine 重组酵母乙型肝炎疫苗
- As leucine zipper is a basic DNA binding motif of many known transcription factors, we examined the transcriptional function of the TCP10L using dual luciferase assay system. 本研究的结果进一步加深了对人体MT基因结构与功能的认识，也为阐明HCC发生的分子机制提供了有重要价值的资料。
- Keywords toxin organic pollutants;bioassay;neutral red;EROD assay;recombinant gene yeast assay;ecological risk assessment; 有毒有机污染物;生物毒性测试;细胞中性红实验;EROD测试;重组基因酵母测试;生态风险;
- Methods The SD rat islets were isolated by digestive method with collagenase and DNase, and incubated in RPMI 1640 culture medium overnight. Multiscreen Assay System was used to observe the effects of CDS on the function of the isolated islets. 方法 胶原酶和DNA酶联合消化法分离SD大鼠胰岛 ,RPMI164 0组织培养液过夜培养 ,采用MilliporeMultiscreen系统观察CDS对胰岛功能的影响。
