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- The mRNA levels of this gene were determined in SCLC cell line SH77 ,big cell lung cancer cell line H460,lung adenocarcinoma cell line SPC A 1 and SPC A 1/CDDP using semi quantitative RT PCR assay. 应用半定量RT-PCR方法检验小细胞肺癌SH77、大细胞肺癌H460、肺腺癌SPC-A-1和SPC-A-1/cDDP等细胞株中该基因mRNA的表达。
- real - time RT PCR 荧光定量RT-PCR
- Establishment and application of quantitative real time PCR assay for detecting duck viral enteritis virus 鸭病毒性肠炎病毒荧光实时定量PCR检测方法的建立和应用
- Real time RT - PCR 实时荧光RT-PCR
- RT PCR was performed for APN gene expression in liver tissue. (2 )APN表达 :根据兔APN基因cDNA序列设计引物 ,提取肝总RNA ,利用RT PCR方法 ,检测肝APNmRNA水平的变化。
- Methods Plasma ETA was measured quantitively by RT PCR. 方法 应用 RT-PCR技术检测外周血循环中 ETA基因表达水平。
- Use RT-PCR and real time PCR to quantify the HLA-G mRNA on deciduas. RT-PCR和real time PCR法检测胎盘蜕膜组织HLA-G mRNA。
- Reverse transcription polymerase chain reaction (RT PCR) assay was used to detect the expression of MDR 1 mRNA and MRP mRNA in 35 patients. Meantime, Immunohistochemistry SABC technique was used to detect P glycoprotein(P gp) in 61 cases. 应用逆转录-多聚酶链式反应(RT-PCR)技术,检测了35 例喉癌患者MDR-1 m RNA、MRPm RNA 基因的表达,同时,应用免疫组化SABC法检测了61 例患者MDR-1 基因产物P-gp。
- The recombination plasmid was transiently transfected into COS7 cells and the expression of the fusion protein was identified by RT PCR, immunofluorescent assay and sandwich ELISA. 以其瞬时转染COS7细胞 ,应用RT PCR、细胞免疫荧光技术 ,及夹心ELISA检测融合蛋白的表达。
- This will be very quick. It will be real time. 它将是非常快捷和实时的。
- Windows NT can be used in real time systems. 窗口新台币能在真正的时间系统中被用。
- Objective To establish a PCR assay for the detection of Helicobacter pylori. 目的建立一种PCR方法检测幽门螺杆菌(Helicobacter pylori,Hp)感染。
- Angiotensin receptor (ATR) mRNA expression of VSMC was measured by RT PCR. RT PCR检测VSMC血管紧张素受体 (ATR)mRNA表达。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- Fabrication process control in real time. 同步的制造程序控制。
- Objective To establish PCR assay of Porcine Parvovirus detection in Trypsin. 目的建立原材料胰蛋白酶中猪细小病毒的PCR检测方法。
- This box will have the entire routine in real time. 这个屏幕将展示出这个流程的真正的时间。
- The expression of cyclin D1 and CDK4 were detected by RT PCR method. RT PCR检测细胞周期素D1(cyclinD1)、细胞周期蛋白依赖激酶 4 (CDK4 )mR NA的表达。
- Non- parameters adaptive real time control. 非参数自适应实时控制。
- The images are created in real time. 这些图像是马上创作出来的。